Name: 核糖核酸酶A 来源于牛胰腺(无DNase & Protease)
SKU: R128619
Availability: InStock

概述规格储存与运输技术文档高级数据关联靶点（人）作用机制质检证书（CoA,COO,BSE/TSE 和分析图谱) 此产品的引用文献技术文档和文章溶液计算器

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为什么选择此级别

ActiBioPure™,EnzymoPure™,天然,无DNA酶,无蛋白酶,生物活性,高性能级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 37 篇同行评审文献引用。

概述

Pancreatic ribonuclease (RNase I) catalyzes cleavage of the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide forming a 2',3'-cyclic phosphate which may then be hydrolyzed to the corresponding 3'-nucleoside phosphate. Ribonuclease A has a molecular weight of 13,700 daltons. It operates in an optimum pH range of 7.0-7.5. Ribonuclease B has a molecular weight of 14,700 ± 300 daltons. It is a glycoprotein which possesses an amino acid composition indistinguishable from that of RNase A. It contains 6 residues of mannose and 2 residues of N-acetylglucosamine per molecule. It is a glycosylated derivative of RNase A. Ribonuclease is inhibited by heavy metal ions and it is competitively inhibited by DNA. Since Molecular Biology Grade RNase A (Product R128619) is essentially free of DNase and protease activities, this product is useful in removing RNA from DNA in nucleic acid work and where other enzymes are used or where intact proteins must be recovered.Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.

Pancreatic ribonuclease (RNase I) catalyzes cleavage of the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide forming a 2',3'-cyclic phosphate which may then be hydrolyzed to the corresponding 3'-nucleoside phosphate. Ribonuclease A has a molecular weight of 13,700 daltons. It operates in an optimum pH range of 7.0-7.5. Ribonuclease B has a molecular weight of 14,700 ± 300 daltons. It is a glycoprotein which possesses an amino acid composition indistinguishable from that of RNase A. It contains 6 residues of mannose and 2 residues of N-acetylglucosamine per molecule. It is a glycosylated derivative of RNase A. Ribonuclease is inhibited by heavy metal ions and it is competitively inhibited by DNA. Since Molecular Biology Grade RNase A (Product R128619) is essentially free of DNase and protease activities, this product is useful in removing RNA from DNA in nucleic acid work and where other enzymes are used or where intact proteins must be recovered.
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.

规格

产品名称

核糖核酸酶A 来源于牛胰腺(无DNase & Protease)

英文别名

规格或纯度

生物活性,ActiBioPure™,天然,高性能,EnzymoPure™,无DNA酶,无蛋白酶,≥2,000 units/mg protein

生化机理

Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA. Catalytic activity: an [RNA] containing cytidine + H2O = an [RNA]-3'-cytidine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA]. EC:4.6.1.18. an [RNA] containing uridine + H2O = an [RNA]-3'-uridine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA]. EC:4.6.1.18.

生物活性

≥2000 U/mg protein

种属

牛(Bovine)

来源

天然

CAS编号和信息

9001-99-4

酶学委员会编号

4.6.1.18

分子类型

酶

储存与运输

物理形态

液体

储存缓冲液

A solution containing approximately 5 mg/ml in 50% Glycerol.

浓度

≥2,000 units/mg protein

储存条件

-20°C储存

运输条件

超低温运输

单位定义

One Kalnitsky Unit causes an increase in absorbance of 1.0 at 260nm at 37°C and pH 5.0 when yeast ribosomal RNA is hydrolyzed to acid soluble oliognucleotides. One Kunitz unit equals 50 units.

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

查看数据表 →

🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

关联靶点（人）

ACHN (49357 活性数据)

点击查看此靶标相关信息

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

CAKI-1 (44928 活性数据)

点击查看此靶标相关信息

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

CCRF-CEM (65223 活性数据)

点击查看此靶标相关信息

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

COLO 205 (50209 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

DU-145 (51482 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

HL-60 (67320 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

HT-29 (80576 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

K562 (73714 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

KM12 (47707 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

M14 (47487 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

MCF7 (126967 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

MOLT-4 (49676 活性数据)

点击查看此靶标相关信息

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

OVCAR-3 (48710 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

OVCAR-4 (44535 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

OVCAR-5 (45555 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

OVCAR-8 (47708 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

PC-3 (62116 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

RPMI-8226 (44974 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

SF-295 (48000 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

SK-MEL-2 (46422 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

SK-MEL-28 (48833 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

SK-MEL-5 (47095 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

SN12C (47755 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

SNB-19 (46794 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

TK-10 (45540 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

U-251 (51189 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

UACC-257 (46019 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

UO-31 (46270 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

786-0 (47912 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

A549 (127892 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

NCI/ADR-RES (33767 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

T47D (39041 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

EKVX (44102 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

NCI-H322M (45589 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

HCC 2998 (41480 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

HCT-116 (91556 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

HOP-92 (41141 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

Hs-578T (29457 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

NCI-H460 (60772 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

SF-268 (49410 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

IGROV-1 (47897 活性数据)

点击查看此靶标相关信息

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

LOX IMVI (44321 活性数据)

点击查看此靶标相关信息

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

HOP-62 (47048 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

MDA-MB-435 (38290 活性数据)

点击查看此靶标相关信息

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

MDA-N (28205 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

Malme-3M (44254 活性数据)

点击查看此靶标相关信息

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

MDA-MB-231 (73002 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

SNB-75 (44215 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

NCI-H226 (44470 活性数据)

点击查看此靶标相关信息

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

NCI-H23 (49055 活性数据)

点击查看此靶标相关信息

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到7个结果

批号(Lot Number)	证书类型	货号
I2615247	分析证书	R128619
E2621773	分析证书	R128619
E2621558	分析证书	R128619
B2626673	分析证书	R128619
C23241200	分析证书	R128619
L1813073	分析证书	R128619
L1813074	分析证书	R128619

技术文档和文章

CRISPR-Cas9和靶向基因组编辑

目录：技术文章

探索主题:

核酸酶细胞和分子生物学研究靶向效率脱靶突变

采用碘化丙啶DNA染色法进行流式细胞术细胞周期分析

目录：实验方案（Protocols）

探索主题:

流式抗体溴化丙锭细胞周期

CUT&RUN-seq实验方案

目录：实验方案（Protocols）

探索主题:

核酸酶 ChIP CUT&RUN ChIC DNA-蛋白质互作 MNase 刀豆球蛋白A ConA磁珠

细胞凋亡DNA片段化分析实验方案

目录：实验方案（Protocols）

探索主题:

凝胶电泳核酸酶细胞凋亡 RNase 凋亡细胞 DNA片段化组织解离酶

双交联ChIP方案

目录：实验方案（Protocols）

探索主题:

抗体核酸酶免疫沉淀交联 ChIP 蛋白酶K 双交联@甲醛 EGS 蛋白-DNA互作

Dual Cross-linking ChIP Protocol

目录：实验方案（Protocols）

探索主题:

Antibody Nuclease Immunoprecipitation Cross-linking ChIP Proteinase K EGS double cross-linking@formaldehyde protein-DNA interaction

蛋白酶K与核糖核酸酶A

目录：技术文章

探索主题:

蛋白酶K 核糖核酸酶A

Proteinase K and Ribonuclease A

目录：技术文章

探索主题:

Proteinase K Ribonuclease A

质粒的五个关键结构特征

目录：技术文章

探索主题:

Plasmids 基因功能解析

Five Key Structural Features of Plasmids

目录：技术文章

探索主题:

质粒 Gene Function Analysis

Proteinase K常见问题

目录：常见问题(FAQs)

探索主题:

Proteinase K 广谱丝氨酸蛋白酶

Proteinase K: Frequently Asked Questions

目录：常见问题(FAQs)

探索主题:

Proteinase K

白介素29（IL-29/IFN-λ1）：分子特征、信号网络与科研应用体系

目录：技术文章

探索主题:

JAK-STAT IL-29 IFN-λ1 Ⅲ型干扰素 IFNLR1/IL-28RA IL-10RB ISGF3 ISG SOCS USP18 黏膜免疫上皮屏障抗病毒

Interleukin-29 (IL-29/IFN-λ1): Molecular Features, Signaling Networks, and a Systematic Framework for Research Applications

目录：技术文章

探索主题:

JAK-STAT IL-29 IFN-λ1 IFNLR1/IL-28RA IL-10RB ISGF3 ISG SOCS USP18 type III interferon mucosal immunity epithelial barrier antiviral

双链脱氧核糖核酸酶（dsDNase）介导的双链DNA水解：酶学特征、方法学策略与生物分析应用综述

目录：技术文章

探索主题:

生物制药样本前处理双链脱氧核糖核酸酶 dsDNase dsDNA水解核酸去除污染控制

dsDNase-Mediated Hydrolysis of Double-Stranded DNA: Enzymatic Characteristics, Methodological Strategies, and Bioanalytical Applications

目录：技术文章

探索主题:

Biopharmaceuticals dsDNase double-stranded deoxyribonuclease dsDNA hydrolysis nucleic-acid removal sample pretreatment contamination control

二苯胺的理化特性、分析应用及其与DNA显色反应综述

目录：技术文章

探索主题:

质量控制二苯胺 Diphenylamine 芳香胺 DNA比色法脱氧核糖糠醛衍生物显色反应

Physicochemical Properties of Diphenylamine, Analytical Applications, and a Review of Its DNA Colorimetric Reaction

目录：技术文章

探索主题:

Quality control Diphenylamine DPA Aromatic amine DNA colorimetric assay Deoxyribose Furfural derivatives Chromogenic reaction

细胞核酸代谢与调控酶在基因表达、RNA稳态与免疫防御中的功能体系综述

目录：技术文章

探索主题:

质量控制先天免疫核酸代谢核酸调控酶基因表达 RNA稳态 RNA加工 RNA降解 RNA编辑核酸感知

A Functional Systems Review of Cellular Nucleic Acid Metabolism and Regulatory Enzymes in Gene Expression, RNA Homeostasis, and Immune Defense

目录：技术文章

探索主题:

RNA degradation Quality control innate immunity nucleic acid metabolism nucleic acid regulatory enzymes gene expression RNA homeostasis RNA processing RNA editing nucleic acid sensing

葡聚糖凝胶的结构特征、操作方法及分离应用

目录：技术文章

探索主题:

葡聚糖凝胶凝胶过滤交联葡聚糖分子筛分脱盐缓冲液置换层析填料

Structural Characteristics, Operational Methods, and Separation Applications of Dextran Gels

目录：技术文章

探索主题:

dextran gel gel filtration crosslinked dextran molecular sieving desalting buffer exchange chromatographic packing material

核酸酶在核酸样本处理、污染控制与分子实验质控中的应用

目录：技术文章

探索主题:

核酸酶 Rt-qPCR MNase DNase RNase NGS qPCR Benzonase 核酸样本处理 DNA污染控制 RNA污染控制分子实验质控核酸降解

Application of Nucleases in Nucleic Acid Sample Processing, Contamination Control, and Molecular Experiment Quality Control

目录：技术文章

探索主题:

Nuclease Rt-qPCR MNase DNase RNase NGS qPCR Benzonase nucleic acid sample processing DNA contamination control RNA contamination control molecular experiment quality control nucleic acid degradation

CRISPR/Cas9 and targeted genome editing

目录：技术文章

探索主题:

Nuclease Cell and Molecular Biology Research Targeting efficiency Off target mutation

Flow cytometry cell cycle analysis using propidium iodide DNA staining

目录：实验方案（Protocols）

探索主题:

Propidium Iodide Flow Cytometry Antibody Cell Cycle

CUT&RUN-seq protocol

目录：实验方案（Protocols）

探索主题:

Nuclease ChIP CUT&RUN ChIC MNase DNA-protein interaction Concanavalin A ConA magnetic beads

Apoptosis DNA Fragmentation Analysis Protocol

目录：实验方案（Protocols）

探索主题:

Gel electrophoresis Nuclease Apoptosis RNase Apoptotic cells DNA fragmentation Tissue dissociation enzyme

RNA结构解析中核酸酶探针怎么选？RNase T1、RNase A、RNase V1与S1核酸酶比较

目录：技术文章

探索主题:

RNA结构解析核酸酶探针 RNase T1 RNase A RNase V1 S1核酸酶 RNA二级结构 RNA footprinting 结构探针 RNA酶切

How to Select Nuclease Probes for RNA Structure Analysis? Comparison of RNase T1, RNase A, RNase V1, and S1 Nuclease

目录：技术文章

探索主题:

RNase T1 RNase A RNase V1 RNA footprinting RNA structure analysis S1 nuclease RNA secondary structure RNA nuclease digestion Nuclease Probes Structural probes

RNA-DNA杂合体如何影响基因组稳定性：RNase H1、RNase H2与R-loop调控机制

目录：技术文章

探索主题:

基因组稳定性 RNA-DNA杂合体 R-loop RNase H1 RNase H2 转录复制冲突复制压力核糖核苷酸切除修复 DNA损伤 DRIP-qPCR

How RNA-DNA Hybrids Affect Genome Stability: RNase H1, RNase H2, and R-loop Regulatory Mechanisms

目录：技术文章

探索主题:

genome stability R-loop RNase H1 RNase H2 DRIP-qPCR RNA-DNA hybrid DNA damage Transcription-Replication Conflict Replication Stress Ribonucleotide Excision Repair

分子克隆筛选与鉴定技术：阳性克隆确认、酶切图谱与测序验证

目录：技术文章

探索主题:

载体构建蓝白斑筛选分子克隆筛选阳性克隆鉴定菌落PCR 酶切鉴定质粒测序

Molecular Cloning Screening and Identification Technologies: Positive Clone Confirmation, Restriction Mapping, and Sequencing Validation

目录：技术文章

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Vector construction Molecular Cloning Screening Positive Clone Identification colony PCR Restriction Digestion Identification Plasmid Sequencing Blue-White Screening

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