Pancreatic ribonuclease (RNase I) catalyzes cleavage of the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide forming a 2',3'-cyclic phosphate which may then be hydrolyzed to the corresponding 3'-nucleoside phosphate. Ribonuclease A has a molecular weight of 13,700 daltons. It operates in an optimum pH range of 7.0-7.5. Ribonuclease B has a molecular weight of 14,700 ± 300 daltons. It is a glycoprotein which possesses an amino acid composition indistinguishable from that of RNase A. It contains 6 residues of mannose and 2 residues of N-acetylglucosamine per molecule. It is a glycosylated derivative of RNase A. Ribonuclease is inhibited by heavy metal ions and it is competitively inhibited by DNA. Since Molecular Biology Grade RNase A (Product R128619) is essentially free of DNase and protease activities, this product is useful in removing RNA from DNA in nucleic acid work and where other enzymes are used or where intact proteins must be recovered.Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.

Pancreatic ribonuclease (RNase I) catalyzes cleavage of the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide forming a 2',3'-cyclic phosphate which may then be hydrolyzed to the corresponding 3'-nucleoside phosphate. Ribonuclease A has a molecular weight of 13,700 daltons. It operates in an optimum pH range of 7.0-7.5. Ribonuclease B has a molecular weight of 14,700 ± 300 daltons. It is a glycoprotein which possesses an amino acid composition indistinguishable from that of RNase A. It contains 6 residues of mannose and 2 residues of N-acetylglucosamine per molecule. It is a glycosylated derivative of RNase A. Ribonuclease is inhibited by heavy metal ions and it is competitively inhibited by DNA. Since Molecular Biology Grade RNase A (Product R128619) is essentially free of DNase and protease activities, this product is useful in removing RNA from DNA in nucleic acid work and where other enzymes are used or where intact proteins must be recovered.
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.

规格

产品名称

核糖核酸酶A 来源于牛胰腺(无DNase & Protease)

英文别名

规格或纯度

生物活性,ActiBioPure™,天然,高性能,EnzymoPure™,无DNA酶,无蛋白酶,≥2,000 units/mg protein

生化机理

Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA. Catalytic activity: an [RNA] containing cytidine + H2O = an [RNA]-3'-cytidine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA]. EC:4.6.1.18. an [RNA] containing uridine + H2O = an [RNA]-3'-uridine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA]. EC:4.6.1.18.

生物活性

≥2000 U/mg protein

种属

牛(Bovine)

来源

天然

CAS编号和信息

9001-99-4

酶学委员会编号

4.6.1.18

分子类型

酶

储存与运输

物理形态

液体

储存缓冲液

A solution containing approximately 5 mg/ml in 50% Glycerol.

浓度

≥2,000 units/mg protein

储存条件

-20°C储存

运输条件

超低温运输

单位定义

One Kalnitsky Unit causes an increase in absorbance of 1.0 at 260nm at 37°C and pH 5.0 when yeast ribosomal RNA is hydrolyzed to acid soluble oliognucleotides. One Kunitz unit equals 50 units.

高级数据

关联靶点（人）

ACHN (49357 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

CAKI-1 (44928 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

CCRF-CEM (65223 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

COLO 205 (50209 活性数据)

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DU-145 (51482 活性数据)

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HL-60 (67320 活性数据)

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HT-29 (80576 活性数据)

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K562 (73714 活性数据)

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KM12 (47707 活性数据)

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M14 (47487 活性数据)

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MCF7 (126967 活性数据)

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MOLT-4 (49676 活性数据)

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OVCAR-3 (48710 活性数据)

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OVCAR-4 (44535 活性数据)

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OVCAR-5 (45555 活性数据)

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OVCAR-8 (47708 活性数据)

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PC-3 (62116 活性数据)

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RPMI-8226 (44974 活性数据)

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SF-295 (48000 活性数据)

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SK-MEL-2 (46422 活性数据)

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SK-MEL-28 (48833 活性数据)

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SK-MEL-5 (47095 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

SN12C (47755 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

SNB-19 (46794 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

TK-10 (45540 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

U-251 (51189 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

UACC-257 (46019 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

UO-31 (46270 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

786-0 (47912 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

A549 (127892 活性数据)

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NCI/ADR-RES (33767 活性数据)

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T47D (39041 活性数据)

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EKVX (44102 活性数据)

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NCI-H322M (45589 活性数据)

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HCC 2998 (41480 活性数据)

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HCT-116 (91556 活性数据)

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HOP-92 (41141 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

Hs-578T (29457 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

NCI-H460 (60772 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

SF-268 (49410 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

IGROV-1 (47897 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

LOX IMVI (44321 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

HOP-62 (47048 活性数据)

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MDA-MB-435 (38290 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

MDA-N (28205 活性数据)

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Malme-3M (44254 活性数据)

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MDA-MB-231 (73002 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

SNB-75 (44215 活性数据)

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NCI-H226 (44470 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

NCI-H23 (49055 活性数据)

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作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

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找到7个结果

批号(Lot Number)	证书类型	货号
I2615247	分析证书	R128619
E2621773	分析证书	R128619
E2621558	分析证书	R128619
B2626673	分析证书	R128619
C23241200	分析证书	R128619
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L1813074	分析证书	R128619

技术文档和文章

CRISPR-Cas9和靶向基因组编辑

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核酸酶细胞和分子生物学研究靶向效率脱靶突变

采用碘化丙啶DNA染色法进行流式细胞术细胞周期分析

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流式抗体溴化丙锭细胞周期

CUT&RUN-seq实验方案

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核酸酶 ChIP CUT&RUN ChIC DNA-蛋白质互作 MNase 刀豆球蛋白A ConA磁珠

细胞凋亡DNA片段化分析实验方案

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凝胶电泳核酸酶细胞凋亡 RNase 凋亡细胞 DNA片段化组织解离酶

双交联ChIP方案

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抗体核酸酶免疫沉淀交联 ChIP 蛋白酶K 双交联@甲醛 EGS 蛋白-DNA互作

Dual Cross-linking ChIP Protocol

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Antibody Nuclease Immunoprecipitation Cross-linking ChIP Proteinase K EGS double cross-linking@formaldehyde protein-DNA interaction

蛋白酶K与核糖核酸酶A

目录：技术文章

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蛋白酶K 核糖核酸酶A

Proteinase K and Ribonuclease A

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Proteinase K Ribonuclease A

质粒的五个关键结构特征

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Plasmids 基因功能解析

Five Key Structural Features of Plasmids

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质粒 Gene Function Analysis

Proteinase K常见问题

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Proteinase K 广谱丝氨酸蛋白酶

Proteinase K: Frequently Asked Questions

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Proteinase K

白介素29（IL-29/IFN-λ1）：分子特征、信号网络与科研应用体系

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JAK-STAT IL-29 IFN-λ1 Ⅲ型干扰素 IFNLR1/IL-28RA IL-10RB ISGF3 ISG SOCS USP18 黏膜免疫上皮屏障抗病毒

Interleukin-29 (IL-29/IFN-λ1): Molecular Features, Signaling Networks, and a Systematic Framework for Research Applications

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JAK-STAT IL-29 IFN-λ1 IFNLR1/IL-28RA IL-10RB ISGF3 ISG SOCS USP18 type III interferon mucosal immunity epithelial barrier antiviral

双链脱氧核糖核酸酶（dsDNase）介导的双链DNA水解：酶学特征、方法学策略与生物分析应用综述

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生物制药样本前处理双链脱氧核糖核酸酶 dsDNase dsDNA水解核酸去除污染控制

dsDNase-Mediated Hydrolysis of Double-Stranded DNA: Enzymatic Characteristics, Methodological Strategies, and Bioanalytical Applications

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Biopharmaceuticals dsDNase double-stranded deoxyribonuclease dsDNA hydrolysis nucleic-acid removal sample pretreatment contamination control

二苯胺的理化特性、分析应用及其与DNA显色反应综述

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质量控制二苯胺 Diphenylamine 芳香胺 DNA比色法脱氧核糖糠醛衍生物显色反应

Physicochemical Properties of Diphenylamine, Analytical Applications, and a Review of Its DNA Colorimetric Reaction

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Quality control Diphenylamine DPA Aromatic amine DNA colorimetric assay Deoxyribose Furfural derivatives Chromogenic reaction

细胞核酸代谢与调控酶在基因表达、RNA稳态与免疫防御中的功能体系综述

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Flow cytometry cell cycle analysis using propidium iodide DNA staining

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CUT&RUN-seq protocol

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Nuclease ChIP CUT&RUN ChIC MNase DNA-protein interaction Concanavalin A ConA magnetic beads

Apoptosis DNA Fragmentation Analysis Protocol

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Gel electrophoresis Nuclease Apoptosis RNase Apoptotic cells DNA fragmentation Tissue dissociation enzyme

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RNA degradation substrate specificity RNase T1 RNase A RNA structure analysis RNase H RNase III RNase R Ribonuclease RNase contamination

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