干扰素刺激基因在抗病毒免疫中的作用：RIG-I、MDA5、IFI35与ISG表达调控
干扰素刺激基因（ISG）是细胞抗病毒状态建立的核心执行层。病毒RNA被RIG-I、MDA5等模式识别受体识别后，可经MAVS、TBK1、IRF3/IRF7和NF-κB诱导I型/III型干扰素产生，再通过JAK-STAT通路驱动ISG表达。
主题
JAK-STAT
用于检测、定量和定位抗原与抗体的技术——ELISA、免疫印迹、免疫组化与流式细胞术。以下是我们知识库中标注该主题的实验方案、常见问题与技术文章。
8 项资源 · 实验方案、常见问题与文章
Roles of Interferon-Stimulated Genes in Antiviral Immunity: RIG-I, MDA5, IFI35, and ISG Expression Regulation
Interferon-stimulated genes (ISGs) constitute the core effector layer for establishing the cellular antiviral state. After viral RNA is recognized by pattern recognition receptors such as RIG-I and MDA5, type I/type III interferon production can be induced through MAVS, TBK1, IRF3/IRF7, and ...
干扰素(IFN)通路的类型分层、信号转导与效应网络
干扰素(interferon, IFN)通路是宿主抗病毒防御、先天免疫激活和免疫稳态调控中最核心的信号系统之一。其本质并不是单一配体触发单一受体，而是由“病原识别—IFN诱导—受体转导—干扰素刺激基因(interferon-stimulated genes, ...
Type Stratification, Signal Transduction, and Effector Networks of the Interferon (IFN) Pathway
The interferon (IFN) pathway is one of the central signaling systems in host antiviral defense, innate immune activation, and immune homeostasis. Its biological essence is not a single ligand triggering a single receptor, but a multilayered network composed of “pathogen sensing - IFN induction - ...
Interleukin-29 (IL-29/IFN-λ1): Molecular Features, Signaling Networks, and a Systematic Framework for Research Applications
Interleukin-29 (IL-29) is a key member of the type III interferon family and is also commonly referred to as interferon lambda 1 (IFN-λ1). IL-29 carries both an “IL-series” designation and a clear interferon-like functional identity.
白介素29（IL-29/IFN-λ1）：分子特征、信号网络与科研应用体系
白介素29（Interleukin-29，IL-29）是Ⅲ型干扰素（type III interferon）家族的重要成员，亦常以干扰素λ1（interferon lambda 1，IFN-λ1）命名。
Interleukin Receptor Signaling: Mechanistic Analysis of the JAK-STAT and NF-κB Axes with an Overview of Assays, Experimental Models, and Translational Boundaries
Interleukins constitute a key group of cytokines produced by immune cells as well as multiple tissue-resident cell types. Their biological effects depend on the assembly of specific receptor complexes and the magnitude of downstream signal transduction, which in turn shapes immune-cell ...
白介素受体信号的JAK-STAT及NF-κB轴机制解析与检测模型及转化边界梳理
白介素是一组由免疫细胞及多种组织细胞共同产生的关键细胞因子，其生物学效应依赖特异性受体复合体的组装与信号转导强度，进而通过JAK-STAT及NF-κB等核心通路塑造免疫细胞的分化、扩增、迁移与效应程序，并参与炎症启动与消退、屏障稳态、组织修复、造血调控及肿瘤免疫等过程。由于受体表达谱、微环境刺激与负反馈调控的共同作用，白介素网络呈现显著的情境依赖性与冗余补偿特征，单一因子读数往往同时折射上游刺激来源、受体可用性与并行通路反馈。 ...
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常与免疫学实验一起研究的技术。
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