用于检测、定量和定位抗原与抗体的技术——ELISA、免疫印迹、免疫组化与流式细胞术。以下是我们知识库中标注该主题的实验方案、常见问题与技术文章。
Aging involves persistent dysregulation of nutrient sensing, energy metabolism, protein homeostasis, and chronic inflammation. IIS, mTOR, AMPK, NF-κB, and Sirtuins are interconnected through nodes including AKT, FOXO, ULK1, and NAD⁺ and jointly regulate cell growth, autophagy, stress adaptation, ...
衰老涉及营养感知、能量代谢、蛋白质稳态和慢性炎症的持续失衡。IIS、mTOR、AMPK、NF-κB和Sirtuins通过AKT、FOXO、ULK1及NAD⁺等节点相互连接，共同调控细胞生长、自噬、应激适应和衰老相关分泌表型。
HMGB1将细胞损伤转化为炎症和趋化信号，并通过TLRs、RAGE及CXCR4调控肿瘤免疫、肿瘤干性和治疗耐受。
HMGB1 converts cellular damage into inflammatory and chemotactic signals and regulates tumor immunity, tumor stemness, and therapy tolerance through TLRs, RAGE, and CXCR4.
核因子 κB（nuclear factor kappa-light-chain-enhancer of activated B cells，NF-κB）是细胞应对感染、炎症和应激刺激时最重要的转录调控系统之一。它的核心作用是把细胞外或细胞内的危险信号转化为细胞核内的基因表达反应。
Nuclear factor κB (nuclear factor kappa-light-chain-enhancer of activated B cells, NF-κB) is one of the most important transcriptional regulatory systems by which cells respond to infection, inflammation, and stress stimuli. Its core role is to convert extracellular or intracellular danger ...
CCR5 is one of the important chemokine receptors in macrophages. It primarily recognizes CC chemokines such as CCL3, CCL4, and CCL5, and regulates macrophage recruitment, migration, inflammatory cytokine release, phagocytic function, and tissue microenvironment remodeling through G ...
CCR5是巨噬细胞中重要的趋化因子受体之一，主要识别CCL3、CCL4、CCL5等CC类趋化因子，并通过G蛋白偶联受体信号调控巨噬细胞募集、迁移、炎症因子释放、吞噬功能和组织微环境重塑。
TNF信号通路是炎症反应、细胞命运调控和组织稳态维持中最核心的信号网络之一。其复杂性并不体现于TNF能够诱导炎症这一单一事实，而在于同一配体刺激可在不同受体背景、泛素化状态、细胞代谢条件和死亡检查点约束下，分别导向炎症放大、存活维持、凋亡或坏死性凋亡。 ...
The TNF signaling pathway is one of the most central signaling networks in inflammatory responses, cell fate regulation, and tissue homeostasis maintenance. Its complexity is not reflected in the single fact that TNF can induce inflammation, but in that the same ligand stimulus can, under ...
The RANK signaling pathway is an important component of the TNF receptor superfamily signaling network, with a core axis composed of RANKL, RANK, and OPG.
RANK信号通路是TNF受体超家族信号网络中的重要组成部分，其核心轴线由RANKL、RANK和OPG构成。
TWEAK-Fn14通路是TNF超家族中兼具炎症调节、组织重塑和细胞命运控制特征的一条信号轴。
The TWEAK-Fn14 pathway is a signaling axis within the TNF superfamily that integrates inflammatory regulation, tissue remodeling, and cell fate control.
黄酮类天然产物并不只是一般意义上的自由基清除成分，而是能够同时作用于活性氧生成、氧化还原缓冲、炎症感知、信号转导及炎症执行层的重要调节分子。
Interleukins constitute a key group of cytokines produced by immune cells as well as multiple tissue-resident cell types. Their biological effects depend on the assembly of specific receptor complexes and the magnitude of downstream signal transduction, which in turn shapes immune-cell ...
白介素是一组由免疫细胞及多种组织细胞共同产生的关键细胞因子，其生物学效应依赖特异性受体复合体的组装与信号转导强度，进而通过JAK-STAT及NF-κB等核心通路塑造免疫细胞的分化、扩增、迁移与效应程序，并参与炎症启动与消退、屏障稳态、组织修复、造血调控及肿瘤免疫等过程。由于受体表达谱、微环境刺激与负反馈调控的共同作用，白介素网络呈现显著的情境依赖性与冗余补偿特征，单一因子读数往往同时折射上游刺激来源、受体可用性与并行通路反馈。 ...
常与免疫学实验一起研究的技术。
危险品化学品经营许可证（带存储）