BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
阿拉丁提供 200 nm 葡聚糖羧基磁珠，具有超顺磁性、磁响应速度快、单分散性好、抗非特异性吸附强等特点。磁珠表面具有丰富的羧基，可与蛋白、抗体、肽、寡核苷酸等以共价键进行高载量偶联。磁珠表面修饰葡聚糖分子，在不同缓冲溶液中具有优良的悬浮稳定性、极低的非特异性吸附，能有效确保反应的均一性和检测一致性，可用于免疫检测、亲和纯化、细胞分选等领域。
产品信息
产品名称 | 葡聚糖羧基磁珠 (200nm) |
SEM 粒径 | 约 200 nm |
表面羧基密度 | ≥200 nmol/mg |
表面电位 | ＜-30 mV |
保存条件 | 2-8℃密封保存 |
参考偶联方案，以 SA 为例
A．试剂准备
名称 | 组成 |
偶联缓冲液 | 15 mM MES，pH5.0 |
交联剂 | EDC 溶液（10 mg/mL，偶联缓冲液配制，现配现用） |
封闭液 | 0.015M PBS（pH7.4），含1%（W/V）BSA |
洗涤液1 | 0.1 M CB，pH9.0 |
洗涤液2 | 0.015M PBS（pH7.4），含0.05%（V/V）Tween-20 |
磁珠保存液 | 0.015M PBS（pH7.4），含1%（W/V）BSA，0.05%（V/V）Tween-20，0.1%（V/V）proclin-300（proclin-300为抑菌剂，请根据需要选择） |
B. 磁珠准备及清洗
1、充分混匀磁珠，取10 mg磁珠至离心管中，磁分离弃上清，加入1 mL偶联缓冲液重悬，水浴超声20 s或涡旋混匀，采用偶联缓冲液重复洗涤1次后定容至10 mg/mL（重悬至1 mL）；
C. SA 包被及磁珠封闭
2、涡旋加入1 mg SA，涡旋混匀充分重悬，37℃摇床反应30 min；
3、涡旋加入0.1 mL 10 mg/mL EDC溶液，37℃摇床反应4 h；
4、磁分离，留取上清测SA偶联率（BCA法或其他）；
注：如需要封闭，请按步骤5进行；如无需封闭，请进入步骤6；
5、（可选步骤）加入封闭液彻底重悬磁珠，37℃摇床反应2 h；反应结束，磁分离弃上清，直接进行步骤7；
D. 包被SA后清洗
6、步骤4结束后，加入1 mL洗涤液1重悬磁珠，37℃摇床混匀30 min，磁分离弃上清；
7、加入1 mL洗涤液2重悬磁珠，磁分离弃上清；重复此步骤3次；
8、加入磁珠保存液重悬磁珠，定容至实验所需浓度，保存于4℃备用。
注意事项
1、磁珠取用前应充分混匀，防止取用改变磁珠浓度，避免长时间超声对磁珠表面破坏；
2、磁珠使用前请进行磁分离并用纯水或所用缓冲溶液清洗2-3遍；
3、磁珠使用和保存过程中应避免冻融。
Aladdin offers 200 nm Dextran Carboxyl Magnetic Beads, which feature superparamagnetism, rapid magnetic response, good monodispersity, and low nonspecific adsorption. The bead surface contains abundant carboxyl groups, enabling high-capacity covalent coupling with proteins, antibodies, peptides, oligonucleotides, and other biomolecules. The bead surface is modified with dextran molecules, providing excellent suspension stability and extremely low nonspecific adsorption in various buffer solutions, thereby effectively ensuring reaction uniformity and assay consistency. The beads can be used for immunoassays, affinity purification, cell separation, and other applications.
Product Information
Product Name | Dextran Carboxyl Magnetic Beads (200nm) |
SEM Particle Size | Approx. 200 nm |
Surface Carboxyl Group Density | ≥200 nmol/mg |
Surface Potential | <-30 mV |
Storage Conditions | Store sealed at 2-8°C |
Reference Coupling Procedure, Using SA as an Example
A. Reagent Preparation
Name | Composition |
Coupling Buffer | 15 mM MES, pH 5.0 |
Crosslinking Agent | EDC solution (10 mg/mL, prepared with coupling buffer, freshly prepared before use) |
Blocking Solution | 0.015 M PBS (pH 7.4), containing 1% (W/V) BSA |
Wash Buffer 1 | 0.1 M CB, pH 9.0 |
Wash Buffer 2 | 0.015 M PBS (pH 7.4), containing 0.05% (V/V) Tween-20 |
Magnetic Bead Storage Solution | 0.015 M PBS (pH 7.4), containing 1% (W/V) BSA, 0.05% (V/V) Tween-20, and 0.1% (V/V) proclin-300 (proclin-300 is a bacteriostatic agent; use as needed) |
B. Magnetic Bead Preparation and Washing
1.Thoroughly mix the magnetic beads. Transfer 10 mg of magnetic beads into a centrifuge tube, perform magnetic separation, and discard the supernatant. Add 1 mL of coupling buffer to resuspend the beads. Sonicate in a water bath for 20 s or mix by vortexing. Wash once more with coupling buffer, then adjust the concentration to 10 mg/mL (resuspend to a final volume of 1 mL);
C. SA Coating and Magnetic Bead Blocking
2.Add 1 mg of SA while vortexing, mix thoroughly by vortexing to fully resuspend the beads, and incubate on a shaker at 37°C for 30 min;
3.Add 0.1 mL of 10 mg/mL EDC solution while vortexing, and incubate on a shaker at 37°C for 4 h;
4.Perform magnetic separation and retain the supernatant to determine the SA coupling efficiency (BCA assay or other methods);
Note: If blocking is required, proceed with Step 5; if blocking is not required, proceed directly to Step 6;
5.(Optional step) Add blocking solution to completely resuspend the magnetic beads and incubate on a shaker at 37°C for 2 h. After incubation, perform magnetic separation, discard the supernatant, and proceed directly to Step 7;
D. Washing After SA Coating
6.After completion of Step 4, add 1 mL of Wash Buffer 1 to resuspend the magnetic beads, mix on a shaker at 37°C for 30 min, perform magnetic separation, and discard the supernatant;
7.Add 1 mL of Wash Buffer 2 to resuspend the magnetic beads, perform magnetic separation, and discard the supernatant. Repeat this step 3 times;
8.Add magnetic bead storage solution to resuspend the magnetic beads, adjust to the concentration required for the experiment, and store at 4°C until use.
Precautions
1.Thoroughly mix the magnetic beads before use to prevent changes in bead concentration during sampling. Avoid prolonged sonication, which may damage the bead surface;
2.Before use, perform magnetic separation and wash the magnetic beads 2-3 times with pure water or the buffer solution to be used;
3.Avoid freeze-thaw cycles during use and storage of the magnetic beads.
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