计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥98% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
DY-46-2 is a high potency and selectivity novel non-nucleoside DNA methyltransferase 3A (DNMT3A) inhibitor with an IC 50 value of 0.39 μM
In Vitro
DY-46-2 (0.1-100 μM, 24, 48 and 72 h) has an inhibitory activity of DNMT3A with an IC 50 value of 0.39 μM, which increases linearly with DNA concentration (IDT-01). DY-46-2 (0.1-100 μM, 24, 48 and 72 h) has inhibitory activity against DNMT1, DNMT3B and G9a with IC 50 values of 13.0 μM, 105 μM and ＞500 μM, respectively. DY-46-2 (0.1-100 μM, 24, 48 and 72 h) has cell viability in cancer cells with IC 50 values of 0.7 μM, 0.3 μM, 0.7 μM, 0.5 μM, 2.1 μM, 1.7 μM and 91 μM for THP-1, HCT116, U937, K562, A549, DU145 and PBMC cell, respectively. DY-46-2 (0.1-100 μM, 24, 48 and 72 h) markedly inhibits the proliferation of cancer cells and shows low cytotoxicity in peripheral blood mononuclear cells (PBMCs). DY-46-2 (1 μM, 72 h) obviously decreases DNMT3A protein levels, as well as reactive expression of a silenced TSG (p53) in HCT116 cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: THP-1, HCT116, U937, K562, A549, DU145 and PBMC cell Concentration: 0.1-100 μM Incubation Time: 24, 48 and 72 h Result: Had remarkable inhibitory potency in the dose- and time-dependent manners and no cytotoxicity in non-tumoral PBMCs (IC 50 >100 μM). Cell Proliferation AssayCell Line: THP-1, HCT116, U937, K562, A549, DU145 and PBMC cell Concentration: 0.1-100 μM Incubation Time: 24, 48 and 72 h Result: Exhibited high anti-proliferative activity with a micromolar range cytotoxicity in all cancer cells. Western Blot AnalysisCell Line: HCT116 cells Concentration: 1 μM Incubation Time: 72 h Result: Decreased DNMT3A and p53 protein levels in the HCT116 cells, apparently sufficient to reactive expression of a silenced TSG (p53).
Form:Solid
IC50& Target:DNMT3A 0.39 μM (IC 50 )
规格
规格或纯度
≥98%
英文名称
DY-46-2
生化机理
DY-46-2 是一种高效力、高选择性的新型非核苷 DNA 甲基转移酶 3A (DNMT3A) 抑制剂，其 IC 50 值为 0.39 μM。
储存条件
-20°C储存
运输条件
超低温运输
作用类型
抑制剂
纯度
≥98%
名称和识别符
分子类型
小分子
分子量
446.48
技术文档
高级数据
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