计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,禁止冷冻。低温运输,禁止冷冻 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
UltraBio™ DNA片段分选磁珠是基于固相载体可逆化固定(Solid Phase Reverse Immobilization, SPRI)技术，使用新型核酸纯化介质包被的磁珠，配合优化的缓冲体系，在特定比例的磁珠悬液中分离纯化出特定长度范围核酸片段的产品。适用于二代测序建库过程中DNA片段的分选与纯化，可根据不同的加样条件，得到不同大小的DNA文库。
使用说明：
一、自备试剂和器材
1、80%乙醇
2、洗脱液：10mM Tris-HCl（pH8.0）或无菌水
3、设备：移液器、涡旋振荡器、磁力架、离心管
二、DNA片段分选操作步骤
1、一次结合：将样本加入1.5mL离心管中，参照下表1加入特定体积的磁珠悬液，涡旋混匀后室温静置10min。将离心管置于磁力架上至溶液完全澄清，将上清转移到一新的1.5mL离心管中，弃去磁珠。
注：磁珠悬液加入量=样本体积*第一次结合比率。
2、二次结合：参照下表1，在上述新的离心管中加入特定比率的磁珠悬液，涡旋混匀后室温静置 10min。将离心管置于磁力架上至溶液完全澄清，弃去上清。
注：磁珠悬液加入量=样本体积*第二次结合比率。
3、清洗1：保持离心管固定于磁力架上，加入200μL 80%乙醇，静置1min，无需重悬磁珠，弃去 上清（保持离心管固定于磁力架上）。
4、清洗2：重复步骤3一次。
5、除醇：保持离心管置于磁力架上，静置通风2-5min除醇。
注：除醇过程中请勿过分干燥磁珠，否则会降低纯化效率。
6、洗脱：将离心管从磁力架上取下，加入30μL洗脱液洗脱，涡旋混匀后室温静置10min。
7、转移核酸：将离心管置于磁力架上，静置2-5min，待磁珠完全吸附后将上清转移至新样品管中备用。
| 分选片段大小 | 200-300bp | 300-400bp | 400-500bp | 500-600bp | 600-800bp |
| 第一次结合比率 | 0.80× | 0.70× | 0.60× | 0.55× | 0.50× |
| 第二次结合比率 | 0.20× | 0.20× | 0.20× | 0.15× | 0.15× |
表1 分选DNA片段大小与磁珠悬液加入量比率
三、PCR产物纯化操作步骤
1、结合：向待纯化的PCR产物样本中加入同等样本体积的磁珠悬液，涡旋混匀后，室温静置5min。 将离心管置于磁力架上至溶液完全澄清，弃去上清。
2、清洗1：保持离心管固定于磁力架上，加入200μL 80%乙醇，静置1min，无需重悬磁珠，弃去 上清（保持离心管固定于磁力架上）。
3、清洗2：重复步骤2一次。
4、除醇：保持离心管置于磁力架上，静置通风2-5min除醇。
注：除醇过程中请勿过分干燥磁珠，否则会降低纯化效率。
5、洗脱：将离心管从磁力架上取下，加入20-100μL的洗脱液，涡旋混匀后，室温静置5min。
6、转移核酸：将离心管置于磁力架上，静置2-5min，待磁珠完全吸附后将上清转移至新的离心管中备用。
注意事项：
1、本试剂为磁珠悬液，严禁冷冻、离心，第一次使用前需充分涡旋混匀；
2、建议样本体积至少为100μL，从而降低加样误差；若样本体积不足100μL可用水补足；
3、磁珠使用前须提前半小时取出，平衡至室温；
4、磁珠请勿过分干燥，否则会降低洗脱效率。
UltraBio™ DNA Size Selection Magnetic Beads utilize Solid Phase Reverse Immobilization (SPRI) technology. These beads are coated with a novel nucleic acid purification medium and combined with an optimized buffer system. At specific bead-to-sample ratios, they enable the isolation and purification of nucleic acid fragments within defined size ranges. This product is designed for DNA fragment size selection and purification during next-generation sequencing (NGS) library preparation, allowing users to obtain DNA libraries of varying sizes by adjusting the sample loading conditions.
Instructions for Use:
I. User-Supplied Reagents and Equipment
1.80% Ethanol
2.Elution Buffer: 10 mM Tris-HCl (pH 8.0) or Nuclease-Free Water
3.Equipment: Pipettes, Vortex mixer, Magnetic rack, Microcentrifuge tubes (1.5 mL)
II. DNA Fragment Size Selection Procedure
1.First Binding:
Transfer the sample to a 1.5 mL microcentrifuge tube.
Add the specified volume of Bead Suspension according to Table 1 (Bead Suspension Volume = Sample Volume × First Binding Ratio).
Vortex-mix thoroughly. Incubate at room temperature for 10 minutes.
Place the tube on the magnetic rack until the solution clears completely.
Transfer the supernatant to a new 1.5 mL microcentrifuge tube. Discard the beads.
2.Second Binding:
Add the specified volume of Bead Suspension to the supernatant from Step 1 according to Table 1 (Bead Suspension Volume = Original Sample Volume × Second Binding Ratio).
Vortex-mix thoroughly. Incubate at room temperature for 10 minutes.
Place the tube on the magnetic rack until the solution clears completely. Discard the supernatant.
3.Wash 1:
With the tube on the magnetic rack, add 200 μL of 80% Ethanol. Do NOT resuspend the beads.
Incubate for 1 minute.
Carefully aspirate and discard the supernatant while keeping the tube on the magnetic rack.
4.Wash 2:
Repeat Step 3 once.
5.Ethanol Removal:
Keep the tube on the magnetic rack. Air-dry the beads at room temperature for 2-5 minutes.
Note: Avoid over-drying the beads, as this reduces purification efficiency.
6.Elution:
Remove the tube from the magnetic rack.
Add 30 μL Elution Buffer. Vortex-mix thoroughly to resuspend the beads.
Incubate at room temperature for 10 minutes.
7.Nucleic Acid Recovery:
Place the tube back on the magnetic rack for 2-5 minutes until the solution clears completely.
Carefully transfer the supernatant (containing the purified DNA) to a new tube.
Table 1: DNA Size Selection Ranges and Bead Suspension Addition Ratios
| Size Selection Range | 200-300bp | 300-400bp | 400-500bp | 500-600bp | 600-800bp |
| First Binding Ratio | 0.80× | 0.70× | 0.60× | 0.55× | 0.50× |
| Second Binding Ratio | 0.20× | 0.20× | 0.20× | 0.15× | 0.15× |
III. PCR Product Purification Procedure
1.Binding:
Add an equal volume of Bead Suspension to the PCR product sample.
Vortex-mix thoroughly. Incubate at room temperature for 5 minutes.
Place the tube on the magnetic rack until the solution clears completely. Discard the supernatant.
2.Wash 1:
With the tube on the magnetic rack, add 200 μL of 80% Ethanol. Do NOT resuspend the beads.
Incubate for 1 minute.
Carefully aspirate and discard the supernatant while keeping the tube on the magnetic rack.
3. Wash 2:
Repeat Step 2 once.
Keep the tube on the magnetic rack. Air-dry the beads at room temperature for 2-5 minutes.
Note: Avoid over-drying the beads, as this reduces purification efficiency.
4.Ethanol Removal:
Keep the tube on the magnetic rack. Air-dry the beads at room temperature for 2-5 minutes.
Note: Avoid over-drying the beads, as this reduces purification efficiency.
5.Elution:
Remove the tube from the magnetic rack.
Add 20-100 μL Elution Buffer (user-defined volume). Vortex-mix thoroughly to resuspend the beads.
Incubate at room temperature for 5 minutes.
6.Nucleic Acid Recovery:
Place the tube back on the magnetic rack for 2-5 minutes until the solution clears completely.
Carefully transfer the supernatant (containing the purified PCR product) to a new tube.
Precautions:
1.Storage & Handling: This reagent is supplied as a Bead Suspension. DO NOT freeze or centrifuge the suspension. Vortex-mix thoroughly before initial use.
2.Sample Volume: Minimum recommended sample volume: ≥100 μL (to minimize pipetting error). For samples <100 μL, adjust to 100 μL using Nuclease-Free Water.
3.Pre-Use Equilibration: Equilibrate the Bead Suspension to room temperature (RT) for ≥30 minutes before use.
4.Bead Drying: Avoid over-drying the beads during the drying step, as this will significantly reduce elution efficiency.
规格
别名
NGS片段筛选磁珠
英文别名
DNA Fragselect XP Magnetic Beads
稳定性与储存
Store at 2-8℃ long term (12 months). Do not freeze.
英文名称
UltraBio™ DNA Size Selection Magnetic Beads
储存条件
2-8°C储存,禁止冷冻
运输条件
低温运输,禁止冷冻
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ25F0926443 |
分析证书 | D751553 |
| ZJ25F0926444 |
分析证书 | D751553 |
技术文档和文章
溶液计算器
常见问题
本产品应如何储存？
请于2–8 °C条件下保存。请勿冷冻。冷冻可能对本品造成不可逆的损坏。
本产品如何运输？
本产品采用湿冰冷藏运输。收货后请立即拆包，并按上述储存条件保存。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）