计算溶液所需的质量、体积或浓度。
,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,禁止冷冻。低温运输,禁止冷冻 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
UltraBio™ RNA纯化磁珠是基于固相载体可逆化固定 (Solid phase reverse immobilization, SPRI) 技术,使用新型核酸纯化介质包被的磁珠,配合优化的缓冲体系,用于从各种体系中纯化RNA,可有效去除蛋白、盐离子等杂质,提高RNA纯度。例:用于文库构建实验中RNA的纯化。
使用说明:
自备试剂和器材
1、自备试剂:80%乙醇(用Nuclease-free H2O配制)、Nuclease-free H2O;
2、自备器材:核酸提取仪、离心管或深孔板、涡旋振荡器、移液器、恒温振荡器等。
操作步骤
1、结合:向待纯化的RNA溶液中加入1.8倍样本体积的RNA纯化磁珠,涡旋混匀后室温静置5min。将离心管置于磁力架上至溶液完全澄清,弃去上清。
2、清洗1:保持离心管固定于磁力架上,加入200μL 80%乙醇(用Nuclease-free H2O配制),静 置1min,无需重悬磁珠,弃去上清(保持离心管固定于磁力架上)。
3、清洗2:重复步骤2一次。
4、除醇: 将离心管从磁力架上取下,静置通风2-5min除醇。
注:除醇过程中请勿过分干燥磁珠,否则会降低纯化效率。
5、洗脱:加入适量体积的Nuclease-free H2O洗脱,涡旋混匀后室温静置5min。
6、转移RNA:将离心管置于磁力架上,静置2-5min,待磁珠完全吸附后将上清转移至新Nuclease-free 离心管中备用。
机提-16/32通道核酸提取仪操作步骤
1、上样准备
在96孔板中分别参照下表用量加入每个相应孔位,可同步完成16/32个样品的处理。
| 样品孔位 | 1、7 | 2、8 | 3、9 | 6、12 |
| 试剂 | 待纯化样本+1.8倍样本体积RNA纯化磁珠 | 80%乙醇(200μL) | 80%乙醇(200μL) | Nuclease-free H2O(用量可根据用户需求调整) |
2、上样提取
将准备好的96孔样品板放入核酸提取仪中,并插入磁棒套; 打开仪器的操作程序,调出对应程序,点击运行,开始提取。
3、洗脱转移
仪器运行结束后,将第6列和第12列孔位的洗脱液转移至干净的无核酸酶的离心管中。
机提-96通道核酸提取仪操作步骤
1、上样准备
在96孔板中分别参照下表用量加入每个相应板位,可同步完成96个样品的处理。
| 板位 | 1 | 2 | 3 | 6 |
| 每孔试剂装量 | 待纯化样本+1.8倍样本体积RNA纯化磁珠 | 80%乙醇(200μL) | 80%乙醇(200μL) | Nuclease-free H2O(用量可根据用户需求调整) |
2、上样提取
将准备好的96孔样品板放入核酸提取仪,并插入磁棒套; 打开仪器的操作程序,调出对应程序,点击运行,开始提取。
3、洗脱转移
仪器运行结束后,将洗脱产物封口保存或移至干净的无核酸酶的PCR板中-20℃保存备用。
注意事项:
1、本试剂为磁珠悬液,严禁冷冻、离心,使用前需充分涡旋混匀;
2、使用不含DNA酶和RNA酶的离心管、Tip头等;
3、磁珠使用前须提前半小时取出,平衡至室温;
4、磁珠请勿过分干燥,否则会降低洗脱效率。
UltraBio™ RNA Clean Magnetic Beads based on Solid Phase Reverse Immobilization (SPRI) technology, these beads utilize a novel nucleic acid purification medium coating combined with an optimized buffer system to purify RNA from diverse sources. They efficiently remove protein, salt ions, and other impurities, enhancing RNA purity—ideal for RNA purification in library construction workflows.
User-Supplied Reagents & Equipment
1. Reagents
80% Ethanol (prepared with Nuclease-free H₂O)
Nuclease-free H₂O
2. Equipment
Nucleic acid extraction instrument
Microcentrifuge tubes or deep-well plates
Vortex mixer
Pipettes
Thermostatic shaker
Manual Operation Protocol
1.Binding
Add 1.8× sample volume of RNA Purification Beads to the RNA solution.
Vortex-mix thoroughly and incubate at room temperature for 5 min.
Place the tube on a magnetic rack until the solution clears completely. Discard the supernatant.
2.Wash 1
Keep the tube on the magnetic rack.
Add 200 μL 80% ethanol (prepared with Nuclease-free H₂O).
Incubate for 1 min (do not resuspend beads).
Discard the supernatant (keep tube on the rack).
3.Wash 2
Repeat Step 2.
4.Ethanol Removal
Remove the tube from the magnetic rack.
Air-dry for 2–5 min at room temperature.
Critical: Avoid over-drying beads to maintain purification efficiency.
5.Elution
Add a user-defined volume of Nuclease-free H₂O.
Vortex-mix thoroughly and incubate at room temperature for 5 min.
6.RNA Recovery
Place the tube on the magnetic rack for 2–5 min until the solution clears.
Transfer the supernatant to a new Nuclease-free tube.
Automated Extraction: 16/32-Channel Instrument
1. Plate Setup (96-Well Plate)
| Sample Position | 1, 7 | 2, 8 | 3, 9 | 6, 12 |
| Reagent | Sample + 1.8× sample volume RNA Clean Magnetic Beads | 200 μL 80% ethanol | 200 μL 80% ethanol | Nuclease-free H₂O (volume adjusted as needed) |
2. Extraction Procedure
Load the prepared 96-well plate into the instrument.
Insert magnetic tip sets.
Select and execute the instrument-specific program.
3. Post-Run Processing
Transfer eluted RNA from columns 6 and 12 to clean Nuclease-free tubes.
Automated Extraction: 96-Channel Instrument
1. Plate Setup (96-Well Plate)
|
Note: Simultaneously processes 96 samples.
2. Extraction Procedure
Load the prepared plate into the instrument.
Insert magnetic tip sets.
Execute the instrument-specific program.
3. Post-Run Processing
Seal elution plates for immediate use or transfer RNA to Nuclease-free PCR plates.
Store at -20°C.
Precautions:
Bead Suspension Handling: This reagent is supplied as a bead suspension. DO NOT freeze or centrifuge. Vortex-mix thoroughly before use.
Nuclease-Free Practice: Use DNase/RNase-free consumables: Centrifuge tubes, Pipette tips, Microplates
Pre-Use Equilibration: Equilibrate beads to room temperature (RT) for ≥30 minutes prior to use.
Avoid Over-Drying: During ethanol removal (Step 4): Air-dry beads for ≤5 minutes. Over-drying significantly reduces RNA yield/recovery.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | R751581 | |
| 分析证书 | R751581 |