含金属强化剂的SIGMAFAST™ DAB 已被用于通过免疫组织化学观察肺组织切片、背根神经节(DRG)神经元和前十字韧带(ACL)细胞中胶原蛋白的免疫组织化学染色。通过添加CoCl2可以增强DAB反应,并产生更强的耐酒精色斑。应用:
使用含金属强化剂的SIGMA FAST™ DAB可制备用于检测过氧化物酶活性的活性底物溶液,无需额外的缓冲液或步骤。常用作免疫组织学的沉淀底物。
SIGMA FAST DAB with Metal Enhancer Tablets require no additional buffers or steps to prepare an active substrate solution for detection of peroxidase activity. Commom use is in immunohistology as a precipitating substrate.
Application:
SIGMAFAST DAB with Metal Enhancer has been used in the visualization of lung tissue sections using immunohistochemistry, dorsal root ganglion (DRG) neurons and immunohistochemical staining for collagen in anterior cruciate ligament (ACL) cells.
The DAB reaction is enhanced by the addition of CoCl2 and produces a more intense stain which is resistant to alcohol.