H2606231计算溶液所需的质量、体积或浓度。
基础 Eagle 培养基 (BME)
有货
为什么选择此级别
用于细胞培养 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光,干燥。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Diamond Vitamin Tween® 80 Solution 40x has been used as a supplement in TYI-S33 medium to grow Entamoeba histolytica trophozoites.Diamond Vitamin Tween® 80 Solution 40x has been used as a supplement:to culture Spironucleus salmonicidato culture Giardia intestinalisto culture Entamoeba histolytica trophozoites应用：
Diamond Vitamin Tween® 80 Solution 40X supplement provides nutrients and reduces the biosynthetic burden of cells in vitro. Vitamin supplements enhance the growth and prolong cell viability in cell culture media.
Eagle基础培养基（BME）由Harry Eagle开发。BME配方是在20世纪50年代末进行的大量研究基础上开发的，旨在确定细胞在培养中的基本营养需求和其他关键因素。历史上，BME已被用于研究正常细胞（WI-38）和转化细胞（小鼠和HeLa）在单层生长中的生长反应。它是所有合成细胞培养基中使用最广泛的一种。
每升用量说明：
方法一：为制备100倍储备溶液，请将0.91克物质溶解于800-900毫升去离子水（ddH2O）或BME培养基中，加热并轻轻搅拌直至完全溶解。调整培养基的pH至所需水平。加入适量水使溶液体积达到1升。使用0.22微米滤膜进行过滤灭菌。分装至无菌容器中。切勿高压灭菌。该溶液含有热不稳定化合物，高压灭菌可能会对其造成损害。使用前，请用BME培养基按1:100的比例稀释。
方法二：1. 准备10倍浓缩储备溶液时，向去离子水或BME培养基中加入0.09克。用BME培养基按1:10的比例稀释，2. 要配制1倍工作溶液，请向去离子水或BME培养基中加入0.009克。
Diamond Vitamin Tween 80 Solution 40X supplement provides nutrients and reduces the biosynthetic burden of cells in vitro. Vitamin supplements enhance the growth and prolong cell viability in cell culture media.
Application:
Diamond Vitamin Tween 80 Solution 40x has been used as a supplement in TYI-S33 medium to grow Entamoeba histolytica trophozoites.
Diamond Vitamin Tween 80 Solution 40x has been used as a supplement:
- to culture Spironucleus salmonicida
- to culture Giardia intestinalis
- to culture Entamoeba histolytica trophozoites
Eagle’s Basal Medium (BME) was developed by Harry Eagle. BME forrmula was developed as a result of numerous studies carried out in the late 1950’s to determine the essential nutritional requirements and other factors critical to the growth of cells in culture. Historically, BME has been used in studies conducted to measure the growth response of normall (WI-38) and transformed (mouse and HeLa) cells in monolayer growth. It is one of the most widely used of all synthetic cell culture media.
Directions per Liter：
In method one,To prepare a 100X stock solution, dissolve 0.91g in 800-900ml of ddH2O or BME media, stirring gently with heat until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving. Dilute 1:100 with BME media prior to use.
Method two: 1. To prepare 10X stock solution, add 0.09g to dH2O or BME media. Dilute 1:10 with BME media. 2. To prepare 1X working solution, add 0.009g to dH2O or BME media.
规格
规格或纯度
用于细胞培养,100X Vitamins (Powder)
英文名称
Basal Medium Eagle (BME)
储存条件
2-8°C储存,避光,干燥
运输条件
低温运输
名称和识别符
分子类型
小分子
技术文档
高级数据
产品属性
| pH | 3.5-6.5 |
|---|
质检证书（CoA,COO,BSE/TSE 和分析图谱)
技术文档和文章
溶液计算器
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