MS-grade Glu-C protease specifically cleaves peptide bonds at the C-terminus of glutamic acid (E) or aspartic acid (D) residues. This enzyme is suitable for in-solution digestion and is not recommended for in-gel digestion. It exhibits higher specificity for glutamic acid residues in ammonium bicarbonate or ammonium acetate buffers, while it can cleave both glutamic acid and aspartic acid residues in phosphate buffer.
Reconstitution Solution:
Reconstitute Glu-C protease with ultrapure water.
pH Range:
rGlu-C exhibits optimal activity at pH 4.0–9.0.
Application:
rGlu-C protease specifically cleaves aspartic acid (D) and glutamic acid (E) residues in denatured proteins.
In-Solution Digestion SOP:
Use 50 μg of Glu-C protease at a protein-to-protease ratio (w/w) of 25:1. Incubate overnight at 37°C for 13–16 hours at pH 8.5.
规格
产品名称
内切蛋白酶Glu-C（V8蛋白酶）（质谱级）
规格或纯度
EnzymoPure™,质谱级 (MS),重组,≥99%(HPLC),≥300 U/mg protein
表达系统
E. coli
CAS编号和信息
66676-43-5
酶学委员会编号
EC 3.4.21.19
分子类型
酶
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
浓度
≥300 U/mg protein
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
单位定义
Glu-c白蛋酶每分钟酶切1.0 μmole BOC-L-Glutamic Acid a phenyl Ester的量，反应条件：37℃，波长270nm。
危险品化学品经营许可证（带存储）