计算溶液所需的质量、体积或浓度。
BioReagent,即用型 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
固定的目的在于保存细胞和组织的原有形态结构,固定剂能阻止内源性溶酶体酶对自身组织和细胞的自溶、抑制细菌和霉菌的生长,固定液分为醛类固定液、汞类固定液、醇类固定液、氧化剂类固定液、苦味酸盐类固定液等,较为常用的是醛类中的福尔马林、醇类中的乙醇。
环保组织固定液 (即用型) 主要以植物多糖和聚乙烯醇为主要原料配制,不含甲醛等有害物质,属于无毒环保型固定液,对于常规石蜡切片固定后形态结构清晰、背景对比度高。在免疫组化、分子病理技术等领域,效果优于甲醛类固定液,多数抗体无需抗原修复处理,可减少因固定产生的假阳性现象,固定标本的组织形态学与甲醛固定相同,组织细胞结构清晰,无空泡化和核固缩等缺陷,该固定液优点是为无色、无刺激性和无毒性挥发成分,不含甲醛,可全面替代甲醛成为病理组织细胞实验室的常规组织细胞固定液,最大限度地减少了对病理科和外科等相关人员的健康危害和环境污染,主要用于病理组织学常规制片染色、传统组织细胞化学染色、免疫组织细胞化学和原位分子杂交等其它分子病理学新技术,该固定液适合于绝大多数组织和细胞的固定,是免疫组织化学和培养细胞固定中最常用的固定液之一,它能较好的保护组织和细胞的形态结构以及核酸。该试剂仅用于科研领域,不宜用于临床诊断或其他用途。
操作步骤 (仅供参考):
1、按实验具体要求操作,直接使用无需稀释。
2、一般标本固定时间控制在 1~4 h/mm, 大标本应适当延长固定时间。
3、固定好的组织,可在福尔马林固定液 (10%) 或 70% 乙醇中长期保存。
注意事项:
1、一经开启,储存过久固定效果易下降。
2、避免过度延长固定时间,否则引起生物大分子过度交联,取材厚度不同,固定时间也不同。
3、固定液的容量应足够,一般固定液与组织块的体积比率应大于 10:1。
4、温度对固定的影响很明显,提高温度可以加速固定作用,但温度不宜过高。
5、取出新鲜组织后,应及时固定,无法及时固定时,应保存于生理盐水中及时送检。
6、试剂开封后请尽快使用,以防影响后续实验效果。
39、英文描述
Purpose of fixation is to preserve the original morphological structure of cells and tissues. Fixatives prevent autolysis of tissues and cells by endogenous lysosomal enzymes and inhibit the growth of bacteria and molds. Fixatives are categorized into aldehyde, mercury‑based, alcoholic, oxidizing agent, and picrate fixatives, among others. Formalin (aldehyde‑based) and ethanol (alcohol‑based) are the most commonly used ones.
The environmentally‑friendly tissue fixative (ready‑to‑use) is mainly formulated with plant polysaccharides and polyvinyl alcohol. It contains no harmful substances such as formaldehyde and is a non‑toxic eco‑friendly fixative. It yields well‑defined morphological structures and high background contrast for conventional paraffin‑embedded sections. It outperforms formaldehyde‑based fixatives in immunohistochemistry and molecular pathology. Most antibodies do not require antigen retrieval, which reduces fixation‑induced false‑positive results. The histomorphology of specimens fixed with this product is comparable to that of formaldehyde‑fixed samples, showing clear tissue‑cellular architecture without vacuolization or karyopyknosis. It features colorless appearance and non‑irritating, non‑toxic volatile components. Formaldehyde‑free, it can fully replace formaldehyde as a routine fixative in histopathology and cytopathology laboratories, minimizing health hazards for staff in pathology and surgery departments as well as environmental pollution. It is applicable to routine histopathological sectioning and staining, classical histochemistry and cytochemistry, immunohistochemistry and immunocytochemistry, in‑situ hybridization and other novel molecular pathological techniques. Suitable for fixation of most tissues and cells, it is one of the preferred fixatives for immunohistochemistry and cultured cells, and provides good preservation of cellular‑tissue morphology and nucleic acids. For research use only; not intended for clinical diagnosis or other clinical applications.
Procedures (for reference only):
1. Use directly without dilution according to experimental requirements.
2. Fixation time for general specimens: 1‑4 h per millimeter of tissue thickness; extend duration for large specimens.
3. Post‑fixed tissues can be stored long‑term in 10% formalin or 70% ethanol.
Precautions
1. Fixation performance may decline upon long‑term storage after opening.
2. Avoid excessive fixation duration, which may cause over‑crosslinking of biomacromolecules. Fixation time varies with tissue thickness.
3. Use sufficient volume of fixative; the volume ratio of fixative to tissue block shall be greater than 10:1.
4. Temperature markedly affects fixation. Elevated temperature accelerates fixation, yet excessive temperature should be avoided.
5. Fresh tissues should be fixed promptly. If immediate fixation is unavailable, store tissues in normal saline for timely delivery.
6. Use the reagent promptly after opening to avoid compromised experimental results.