计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥96% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
EDP-305 is an orally active, potent and selective farnesoid X receptor (FXR) agonist, with EC 50 values of 34 nM (chimeric FXR in CHO cells) and 8 nM (full-length FXR in HEK cells). EDP-305 shows a potent and consistent antifibrotic effect. EDP-305 can be used for primary biliary cholangitis (PBC) and non-alcoholic steatohepatitis (NASH) research
In Vitro
EDP‐305 (10 μM, 72 h) directly activates FXR in liver hepatoctyes but not stellate cells. EDP-305 (0-5 μM, 16 h) increases the expression of the FXR target gene, SHP, and downregulates CYP7A1 expression in HepaRG hepatocytes. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot AnalysisCell Line: Hepatic stellate cell (HSC) lines, primary HSCs and hepatocytes Concentration: 10 μM Incubation Time: 72 h Result: Induced mRNA expression of SHP and FGF19 in human hepatocytes, and elicited no induction of downstream targets SHP or FGF15/19 in stellate lines. RT-PCRCell Line: HepaRG hepatocytes Concentration: 0.05, 0.1, 0.5, 1, 5, 10, 50, 100, 500, 1000, 5000 nM Incubation Time: 16 h Result: Dose-dependently increased the expression of the FXR target gene, SHP, and downregulated CYP7A1 expression in HepaRG hepatocytes.
In Vivo
EDP‐305 (0-30 mg/kg, Oral gavage, daily for 2 weeks) reduces serum markers of liver injury, and reduces liver fibrosis in a dose-dependent manner in BDL rats . EDP‐305 (0-30 mg/kg, Oral gavage, daily for 6 weeks) reduces liver fibrosis in a dose-dependent manner in CDAHFD mice . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Male CD rats (underwent BDL, n=24, n=8 for each group) Dosage: 0, 10 and 30 mg/kg Administration: Oral gavage, daily, started on day 4 after BDL and continued until days 17-18 Result: Significantly reduced alanine aminotransferase and aspartate aminotransferase. Showed a dose-dependent reduction in CPA. Reduced hydroxyproline levels in whole liver tissue samples. Reduced messenger RNA (mRNA) relative quantification (RQ) for both Col1a1 and actin, alpha 2, smooth muscle, aorta (Acta2). Animal Model: Male C57BL/6 mice (n = 24, fed a CDAHFD consisting of 60% kcal fat and 0.1% methionine) Dosage: 0, 10 and 30 mg/kg Administration: Oral gavage, daily, started at the beginning of week 6 on the diet and were continued until week 12 Result: Reduced serum triglycerides, and significantly reduced hydroxyproline and MR liver signal intensity in a dose-dependent manner. Showed a dose‐dependent reduction in mRNA expression of lysyl oxidase genes Lox and Loxl1-4.
Form:Solid
IC50& Target:IC50: 8 ± 3 nM (Full-length FXR in HEK cells), 34 ± 8 nM (Chimeric FXR in CHO cells), >15000 nM (TGR5 in CHO cells)
规格
规格或纯度
≥96%
英文名称
EDP-305
生化机理
EDP-305 是一种口服活性、强效和选择性法尼类固醇 X 受体 (FXR) 激动剂，其 EC 50 值为 34 nM（CHO 细胞中的嵌合 FXR）和 8 nM（HEK 细胞中的全长 FXR）。EDP-305 具有强效且持续的抗纤维化作用。EDP-305 可以
储存条件
-20°C储存
运输条件
超低温运输
作用类型
激活剂
纯度
≥96%
名称和识别符
分子类型
小分子
IUPAC Name
1-(4-tert-butylphenyl)sulfonyl-3-[(3R)-3-[(3S,5R,6R,7R,8R,9S,10S,13R,14S,17R)-6-ethyl-3,7-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]butyl]urea
INCHI
1S/C36H58N2O5S/c1-8-26-30-21-24(39)15-18-36(30,7)29-16-19-35(6)27(13-14-28(35)31(29)32(26)40)22(2)17-20-37-33(41)38-44(42,43)25-11-9-23(10-12-25)34(3,4)5/h9-12,22,24,26-32,39-40H,8,13-21H2,1-7H3,(H2,37,38,41)/t22-,24+,26-,27-,28+,29+,30-,31-,32-,35-,36-/m1/s1
InChi Key
SJKLCUGQVVYDCX-YNNXSQSESA-N
Smiles
CCC1C2CC(CCC2(C3CCC4(C(C3C1O)CCC4C(C)CCNC(=O)NS(=O)(=O)C5=CC=C(C=C5)C(C)(C)C)C)C)O
PubChem CID
分子量
630.92
技术文档
高级数据
三维结构
交互式化学结构模型
化学和物理性质
溶解性
DMSO : ≥ 100 mg/mL (158.50 mM)
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
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