计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥99% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
EGFR-IN-11 is a fourth-generation EGFR -tyrosine kinase inhibitor ( EGFR-TKI ) with an IC 50 of 18 nM for triple mutant EGFR L858R/T790M/C797S . EGFR-IN-11 significantly suppresses the EGFR phosphorylation, induce the apoptosis , and arrest cell cycle at G0/G1
In Vitro
EGFR-IN-11 (Compound D9; 0.0001-10 µM; 72 hours) shows significantly potent anti-proliferation against HCC827 and H1975 cell lines with IC 50 s of 0.88 nM and 0.20 µM, respectively. EGFR-IN-11 (0.01-1.00 µM for HCC827 cells; 0.1-10.00 µM for H1975 and A549 cells; 8 hours) suppresses EGFR phosphorylation in a concentration-dependent manner in the HCC827, H1975 and A549 cell line. EGFR-IN-11 (1 µM; 24 h) potently induces the apoptosis of HCC827 cells.EGFR-IN-11 (1 µM; 24 h) induces cell cycle arrests in HCC827 cell. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Proliferation AssayCell Line: Human lung cancer cell lines HCC827 (EGFR Del E746-A750 ), H1975 (EGFR L858R/T790M ) and A549 (EGFRWT); epidermoid carcinoma cell line A431 (EGFRWT) Concentration: 0.0001, 0.0003, 0.001, 0.003, 0.01, 0.1, 1, 10 µM Incubation Time: 72 hours Result: Inhibited HCC827 H1975 A549 cells proliferation with IC 50 s of 0.88±0.09 nM, 0.20±0.01 μM, 2.91±0.61 μM, and >10 μM, respectively. Western Blot AnalysisCell Line: HCC827, H1975 and A549 cells Concentration: 1.00, 0.10 and 0.01 µM for HCC827 cells; 10.00, 1.00 and 0.10 µM for H1975 and A549 cells Incubation Time: 8 hours Result: Suppressed EGFR phosphorylation in a concentration-dependent manner. EGFR phosphorylation in the HCC827 cell line was more remarkably suppressed than in the H1975 and A549 cell lines. Apoptosis AnalysisCell Line: HCC827 cells Concentration: 1 µM Incubation Time: 24 hours Result: The percentages of apoptotic cells is 56.91%. Cell Cycle AnalysisCell Line: HCC827 cells Concentration: 1 µM Incubation Time: 24 hours Result: The number of HCC827 cells in G0/G1 phase was increased significantly.
Form:Solid
IC50& Target:EGFR L858R/T790M/C797S 18 nM (IC 50 )
规格
规格或纯度
≥99%
英文名称
EGFR-IN-11
生化机理
EGFR-IN-11 是第四代表皮生长因子受体酪氨酸激酶抑制剂（EGFR-TKI），对三突变表皮生长因子受体 L858R/T790M/C797S 的 IC50 为 18 nM。EGFR-IN-11能明显抑制表皮生长因子受体磷酸化，诱导细胞凋亡，并使细胞周期停滞于
储存条件
-20°C储存
运输条件
超低温运输
作用类型
抑制剂
纯度
≥99%
名称和识别符
分子类型
小分子
分子量
573.71
技术文档
高级数据
化学和物理性质
溶解性
DMSO : 125 mg/mL (217.88 mM; Need ultrasonic)
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）