计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥98% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
E235 is an expression regulator of activates transcription factor 4 ( ATF4 ). E235 reduces cell viability by activating integrated stress response (ISR) and DNA damage response signals. E235 has anti-proliferative activity and can be used for tumor research
In Vitro
E235 (1 µM; 2, 4, 8, 16 or 24 h) down-regulates the level of XBP-1s mRNA in HT1080 cells with time dependent manner. E235 (0.1-10 µM; 4 d or 5 d) has anti-proliferative activity on HT1080, RPMI-8226, B16F10, 4T1, HT1080 shNT and HT1080 shATF4 cells. E235 (0, 1, 5 and 10 µM; 4 h) increases the expression of p53 with dose-dependent manner in AG1522 cells. E235 (1 µM; 2 h) increases the expression of p-Chk2 in AG1522 cells. E235 (0, 1, 5 and 10 µM; 2 h) increases the expression of p-p53 in AG1522 and HT1080 cells. E235 (0, 0.5, 1, 5 and 10 µM; 0.5 h) increases the expression of γ-H2AX with dose-dependent manner in HT1080 cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot AnalysisCell Line: HT1080, B16F10 and AG1522 cells. Concentration: 0, 1, 5 and 10 µM. Incubation Time: 4 h. Result: Increased the expression of ATF4 with dose-dependent manner. Western Blot AnalysisCell Line: HT1080 cells. Concentration: 0, 0.5, 1, 5 or 10 µM. Incubation Time: 2, 4 or 8 h. Result: Increased the expression of p-elF2α with time and dose dependent manner. Cell Proliferation AssayCell Line: HT1080 or RPMI-8226 cells. Concentration: 1 µM. Incubation Time: 0, 8, 16 and 24 h. Result: Exhibited anti-proliferative activity. Cell Viability AssayCell Line: AG1522 cells. Concentration: 0, 0.1, 0.5, 1 and 10 µM. Incubation Time: 4 d. Result: Inhibited cell viability with dose-dependent manner. Cell Cycle AnalysisCell Line: HT1080 cells and B16F10 cells. Concentration: 1 µM. Incubation Time: 8, 16 and 24 h. Result: Caused cell arrest during G2/M phase.
Form:Solid
IC50& Target:Activates transcription factor 4, ATF4
规格
规格或纯度
≥98%
英文名称
E235
生化机理
E235 是激活转录因子 4（ATF4）的表达调节剂。E235 通过激活综合应激反应（ISR）和 DNA 损伤反应信号来降低细胞活力。E235 具有抗增殖活性，可用于肿瘤研究。
储存条件
-20°C储存
运输条件
超低温运输
作用类型
激活剂
纯度
≥98%
名称和识别符
分子类型
小分子
分子量
484.59
技术文档
高级数据
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