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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
白细胞介素6(IL-6)是一种白细胞介素,它既是促炎症细胞因子,又是抗炎的肌动因子。它是由IL6基因编码的。此外,成骨细胞分泌IL-6来刺激破骨细胞的形成。许多血管内膜的平滑肌细胞也会产生IL-6作为促炎症细胞因子。IL-6作为抗炎肌因的作用是通过其对TNF-α和IL-1的抑制作用以及对IL-1ra和IL-10的激活作用而介导的。IL-6是由巨噬细胞对特定的微生物分子(被称为病原体相关分子模式(PAMPs))作出反应而分泌的。这些PAMPs与先天免疫系统的一组重要检测分子结合,称为模式识别受体(PRRs),包括Toll样受体(TLRs)。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有猴白细胞介素6(IL-6)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的猴白细胞介素6(IL-6)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Monkey IL-6. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Monkey IL-6 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.