BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
I型胶原蛋白是最丰富的胶原蛋白。它形成大的嗜酸性纤维，称为胶原纤维。它存在于疤痕组织，最终产品时组织愈合通过修复、以及腱、韧带、所述内膜的肌原纤维的有机部分骨，所述真皮中、牙质和器官胶囊。它的COL1A1基因产生的亲ALPHA1（I）链。该链与另一条原α1（I）链以及原α2（I）链（由COL1A2基因产生）结合在一起，形成I型原胶原分子。这些三链、绳状前胶原分子必须被细胞外的酶处理。一旦处理了这些分子，它们就会排列成细长的细纤维，这些细纤维在细胞周围的空间中相互交联。交联导致形成非常牢固的成熟的I型胶原纤维。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有大鼠Ⅰ型胶原(COL1)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠Ⅰ型胶原(COL1)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat COL1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat COL1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
危险品化学品经营许可证（带存储）