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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
微管关联蛋白 1 轻链 3β(MAP1LC3b)是一种蛋白质,由 MAP1LC3B 基因编码。它是自噬途径中的一个中心蛋白,它在自噬底物选择和自噬体生物生成中发挥作用。它是最广泛使用的自噬体标志物。它是高度保守的 ATG8 蛋白家族的一个成员。LC3 的脂质修饰形式,称为 LC3-II,被认为参与自噬体膜的扩增和融合事件。LC3 还与自噬受体(例如 SQSTM1)一起在选择性捕获货物以进行自噬降解中发挥作用。独立于自噬体,单个可溶性 LC3 与细胞质中约 500 kDa 的复合物相关。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠微管关联蛋白1轻链3β(MAP1LC3b)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠微管关联蛋白1轻链3β(MAP1LC3b)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat MAP1LC3b. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat MAP1LC3b present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.