BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
线粒体转录因子A(TFAM)是一种蛋白质，由TFAM基因编码。该基因编码一个线粒体转录因子，是线粒体转录的关键激活剂，也是线粒体基因组复制的参与者。它与线粒体启动子DNA结合以帮助线粒体基因组的转录。它是一个双框高移动性组DNA结合和弯曲蛋白。这种弯曲作用在哺乳动物中对线粒体转录的启动很重要，但在酵母的同源物Abf2中却不是。它也可能参与线粒体基因组的包装，因为其结合活性是非序列特异性的。它已被证明与TFB1M和TFB2M相互作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有大鼠线粒体转录因子A(TFAM)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠线粒体转录因子A(TFAM)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat TFAM. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat TFAM present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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