BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
脂蛋白相关磷脂酶A2（Lp-PLA2）也被称为血小板活化因子乙酰水解酶（PAF-AH），是一种磷脂酶A2，由PLA2G7基因编码。Lp-PLA2是一种45kDa的蛋白质，有441个氨基酸。在血液中，Lp-PLA2主要与低密度脂蛋白（LDL）一起流动，不到20%与高密度脂蛋白HDL有关。它是一种由炎症细胞产生的酶，可以水解低密度脂蛋白中的氧化磷脂。Lp-PLA2通过水解sn-2位的乙酰基，催化PAF降解为非活性产品，产生生物活性产品LYSO-PAF和乙酸盐。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有大鼠脂蛋白关联磷脂酶A2(LpPLA2)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠脂蛋白关联磷脂酶A2(LpPLA2)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat LpPLA2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat LpPLA2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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