BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
转酮醇酶，也被称为TKT，是由TKT基因编码的酶。它参与所有生物体中的磷酸戊糖途径和光合作用的卡尔文循环。它催化两个重要的反应，它们在这两个途径中以相反的方向起作用。在非氧化性磷酸戊糖途径的第一个反应中，辅因子二磷酸硫胺素接受来自5-碳酮糖的2-碳片段，然后将该片段转移至5-碳醛糖以形成7-碳酮糖。从D-木酮糖-5-P中提取两个碳得到3-碳醛糖-3-P。在卡尔文循环中，转酮醇酶催化反向反应，将二庚酮糖-7-P和甘油醛-3-P转化为戊糖，醛糖D-核糖-5-P和酮糖D-木酮糖-5-P。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有大鼠转酮醇酶(TKT)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠转酮醇酶(TKT)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat TKT. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat TKT present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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