BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
载脂蛋白A4（也被称为apoA-IV、apoAIV或apoA4）是血浆蛋白，是基因APOA4的产物。APOA4基因的主要翻译产物是一个396个残基的前蛋白，经过蛋白分解处理后产生apo A4，一个376个残基的成熟O型连接糖蛋白。在大多数哺乳动物中，载脂蛋白A-IV的合成局限于肠道；然而在大鼠中也有肝脏合成。APOA4位于11号染色体上，与APOA1和APOC3紧密相连。APOA4包含3个外显子，由两个内含子隔开，并且是多态的，尽管大多数报道的序列多态性发生在第3外显子。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有大鼠载脂蛋白A4(apo-A4)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠载脂蛋白A4(apo-A4)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat apo-A4. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat apo-A4 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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