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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
载脂蛋白B(ApoB)是一种蛋白质,是由APOB基因编码的。该蛋白在血浆中主要有两种异构体:ApoB48和ApoB100。载脂蛋白B-100是载脂蛋白B组中最大的蛋白质,由4563个氨基酸组成。这两种异构体都由APOB和大于16kb的单一mRNA转录本编码。当通过RNA编辑在2153残基上产生一个终止密码子(UAA)时,就会产生ApoB48。并且载脂蛋白B是乳糜微粒、VLDL、Lp(a)、IDL和LDL颗粒的主要载脂蛋白,它负责将脂肪分子(脂质),包括胆固醇,运送到所有组织的所有细胞。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠载脂蛋白B(apo-B)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠载脂蛋白B(apo-B)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat apo-B. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat apo-B present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.