计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
肿瘤蛋白 p53(TP53),也称为 p53,细胞肿瘤抗原 p53,由 TP53 及 Trp53 基因编码。它是最早发现的肿瘤抑制基因所编码的蛋白之一。它能调节细胞 周期,促使细胞出现凋亡或细胞衰老等现象,从而避免细胞癌变发生。它能 保持基因组的稳定性,避免或减少突变的发生。因此被称为基因组守护者, 因为它通过防止基因组突变来保持稳定性,所以被归类为肿瘤抑制基因。p53 是一系列被称为肿瘤抑制蛋白的同源异构蛋白的统称。p53 这个名字是在 1979 年给出的,用来描述表观分子质量;SDS-PAGE 分析表明它是一种 53kDa 的蛋白质。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠p53(p53)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠p53(p53)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse p53. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse p53 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.