计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
乙醛脱氢酶(Acetaldehyde dehydrogenase),缩写ALDH,它是醛脱氢酶的一种,负责催化乙醛氧化为乙酸的反应,肝中的乙醇脱氢酶负责将乙醇(酒的成分)氧化为乙醛,生成的乙醛作为底物进一步在乙醛脱氢酶催化下转变为无害的乙酸(即醋的成分)。醛脱氢酶是一种多态性酶,负责将醛氧化成羧酸,离开肝脏后由身体的肌肉和心脏代谢。这些酶在哺乳动物中有三个不同的类别:第一类(低 Km,细胞膜),第二类(低 Km,线粒体),以及第三类(高 Km,如在肿瘤、胃和角膜中表达的那些)。在这三类中,存在构成性和诱导性形式。ALDH1和 ALDH2是最重要的醛氧化酶,都是由54 kDa亚单位组成的四元酶。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠乙醛脱氢酶(ALDH)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠乙醛脱氢酶(ALDH)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse ALDH. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse ALDH present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.