BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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天门冬氨酸氨基转移酶（AST）或天门冬氨酸转氨酶，又称AspAT/ASAT/AAT或（血清）谷氨酸草酰乙酸转氨酶（GOT，SGOT），是一种依赖磷酸吡哆醛（PLP）的转氨酶（AST催化天门冬氨酸和谷氨酸之间α-氨基的可逆转移，因此它是氨基酸代谢中的一个重要酶。AST存在于肝脏、心脏、骨骼肌、肾脏、大脑和红血球中。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠天门冬氨酸氨基转移酶(AST)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠天门冬氨酸氨基转移酶(AST)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse AST. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse AST present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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