计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
聚集蛋白聚糖(Aggrecan),也被称为软骨特异性蛋白多糖核心蛋白 (CSPCP)或硫酸软骨素蛋白多糖 1,是一种由 ACAN 基因编码的蛋白质。 该基因是 clockican(硫酸软骨素蛋白多糖)家族的成员。所编码的蛋白是 软骨组织中细胞外基质的一个组成部分,它能承受软骨的压缩。Aggrecan 是一种蛋白多糖,或者说是一种用大型碳水化合物修饰的蛋白质;该蛋白质 的形式长 2316 个氨基酸,由于替代拼接,可以表达为多种异构体。它也是 一种高分子量蛋白多糖。它表现出一种瓶状结构,其中硫酸软骨素和硫酸角 质素糖胺聚糖(GAG)链连接到一个扩展的蛋白质核心。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠聚集蛋白聚糖(AGC)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠聚集蛋白聚糖(AGC)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse AGC. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse AGC present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.