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花生四烯酸 12 脂氧合酶(ALOX12)又称 12-脂氧酶、12S-脂氧酶、12-LOX 和 12S-LOX，是一种脂氧酶类型的酶，由 ALOX12 基因编码，该基因与其他脂氧酶一起位于染色体 17p13.3 上。它由 663 个氨基酸组成。它最初被称为花生四烯酸 12-脂氧合酶，由诺贝尔奖获得者 Bengt I. Samuelsson 和他著名的同事 Mats Hamberg 于 1974 年首次确定其特征。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠花生四烯酸12脂氧合酶(ALOX12)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠花生四烯酸12脂氧合酶(ALOX12)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse ALOX12. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse ALOX12 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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