计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Parkin 蛋白(PP)是一种蛋白质,由 PARK2 基因编码。它是一种多蛋白 E3 泛 素连接酶复合物的组成部分,该复合物又是泛素-蛋白酶体系统的一部分,该 系统介导蛋白质的靶向降解。该基因的突变会导致一种家族性的帕金森病, 即常染色体隐性少年帕金森病(AR-JP)。此外,Parkin 被描述为丝裂吞噬 (线粒体的自噬)所必需。此外,Parkin 含有一个 C 端图案,可与 PDZ 结 构域结合。它已被证明与含有 PDZ 结构域的蛋白 CASK 和 PICK1 以 PDZ 依 赖的方式结合。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人Parkin蛋白(PP)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人Parkin蛋白(PP)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human PP. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human PP present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.