计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
α-2-巨球蛋白(α2M)是一种在血液中发现的大型(720 KDa)血浆蛋白。它主要由肝脏产生,也可由巨噬细胞、成纤维细胞和肾上腺皮质细胞局部合成。在人类中,它是由A2M基因编码的。α2巨球蛋白作为一种抗蛋白酶,能够使大量的蛋白酶失活。它通过抑制血浆蛋白和卡利克蛋白而发挥纤维蛋白溶解抑制剂的作用。它通过抑制凝血酶发挥凝血抑制剂的作用。α-2-巨球蛋白可能作为一种载体蛋白,因为它还与许多生长因子和细胞因子结合,如血小板衍生的生长因子、碱性成纤维细胞生长因子、TGF-β、胰岛素和IL-1β。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人α2巨球蛋白(α2-MG)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人α2巨球蛋白(α2-MG)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human α2-MG. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human α2-MG present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.