BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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Ⅹ组磷脂酶A2(PLA2G10)这种依赖钙的酶水解甘油磷脂，产生游离脂肪酸和溶血磷脂。这种酶催化从细胞膜磷脂中释放花生四烯酸，从而在各种炎症脂质介质，如前列腺素的产生中发挥作用。它可能通过其在脂质代谢中的作用促进乳腺癌细胞的生存。与它相关的疾病包括急性胸腔综合征和镰状细胞病。其相关途径包括甘油磷脂的生物合成和PE的酰基链重塑。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人Ⅹ组磷脂酶A2(PLA2G10)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人Ⅹ组磷脂酶A2(PLA2G10)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human PLA2G10. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human PLA2G10 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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