计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
凝血酶/抗凝血酶复合体(TAT)是凝血酶和抗凝血酶的蛋白复合物,是凝血功能净激活的标志。TAT 的形成是对血管破裂后凝血引起的高凝血酶水平的反应。由于凝血酶会迅速被抗凝血酶结合,因此 TAT 是衡量血液中凝血酶水平的一个有用指标。凝血酶可以通过血脑屏障,破坏神经元并可能引起脑水肿。TAT 的半衰期约为 15 分钟。TAT 水平随着怀孕和含炔雌醇的避孕药而增加。据报道,绝经期激素治疗也会增加它们,尽管结果喜忧参半,以及大剂量肠外雌二醇治疗前列腺癌。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人凝血酶抗凝血酶复合物(TAT)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人凝血酶抗凝血酶复合物(TAT)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human TAT. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human TAT present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.