BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
嘌呤霉素敏感性氨肽酶也被称为细胞膜丙氨酸氨基肽酶或丙氨酸氨基肽酶，是一种在人类中由NPEPPS基因编码的酶。该基因编码嘌呤敏感氨肽酶，是一种锌金属肽酶，可水解其底物N端的氨基酸。该蛋白已被定位到细胞质和细胞膜上。这种酶在大脑中降解脑啡肽，参与调节细胞周期的蛋白水解事件。它已被确定为TAU诱导的神经变性的新型调节剂，通过直接蛋白水解TAU蛋白而具有神经保护作用。它被用作检测肾脏损害的生物标志物，可用于帮助诊断某些肾脏疾病。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人嘌呤霉素敏感性氨肽酶(PSA)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人嘌呤霉素敏感性氨肽酶(PSA)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human PSA. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human PSA present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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