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白三烯 A4 水解酶,也被称为 LTA4H,由 LTA4H 基因编码。该基因编码的蛋白质是一种含有水解酶和氨基肽酶活性的双功能酶,它将白三烯 A4 转化成白三烯 B4 并充当氨肽酶。这种酶属于水解酶家族,特别是那些作用于醚键 (醚水解酶)的酶。该酶类的系统名称是(7E,9E,11Z,14Z)-(5S,6S) -5,6-环氧二氧化-7,9,11,14-四烯酸酯水解酶。这种酶参与花生四烯酸 代谢。已经为该基因发现了几种编码不同亚型的转录本变体。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人白三烯A4水解酶(LTA4H)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人白三烯A4水解酶(LTA4H)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human LTA4H. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human LTA4H present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.