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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
白介素2受体α链是一种由IL-2RA基因编码的蛋白质。白细胞介素2受体α和β链与共同的γ链一起构成高亲和力IL2受体。同二聚体的α链产生低亲和力受体，而同二聚体的β链产生中等亲和力受体。通常，可溶性IL2RA是一种完整的膜蛋白，已被分离并确定为由细胞外蛋白水解引起的。交替剪接的IL2RA mRNA已被分离，但目前尚不清楚每种mRNA的重要性。它是一种I型跨膜蛋白，存在于活化的T细胞、活化的B细胞、一些胸腺细胞、骨髓前体和少突胶质细胞。它在大多数B细胞肿瘤、一些急性非淋巴细胞白血病、神经母细胞瘤、肥大细胞病和肿瘤浸润性淋巴细胞中表达。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人白细胞介素2受体α链(IL-2Rα;CD25)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人白细胞介素2受体α链(IL-2Rα;CD25)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human IL-2Rα;CD25. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human IL-2Rα;CD25 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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