BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
人磷脂酶A1(PL-A1)由PLA1A基因编码，是一种去除1-酰基的磷脂酶。可将磷脂水解为脂肪酸。 磷脂酶A1（PL-A1）专门催化磷脂的SN-1位裂解，形成脂肪酸和溶血磷脂 。PLA1存在于包括人类在内的众多物种中，具有多种细胞功能，包括调节和促进溶血磷脂介质的产生，并充当消化酶。这些酶负责细胞磷脂的快速周转率。除此之外，PL-A1催化的反应产物是一种脂肪酸和一种溶血磷脂，对各种生物功能，如血小板聚集和平滑肌收缩非常重要。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人磷脂酶A1(PL-A1)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人磷脂酶A1(PL-A1)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human PL-A1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human PL-A1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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