计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
磷酸甘油酸酯激酶 1,也被称为 PGK1,是由PGK1 基因编码的酶。该基因编码的蛋白质是一种糖酵解酶,催化 1,3-二磷酸甘油酯向3-磷酸甘油酯的转化。编码的蛋白质也可以作为聚合酶α的辅助因子。此外,这种蛋白质由肿瘤细胞分泌,在那里它通过减少丝氨酸蛋白酶,纤溶酶中的二硫键来参与血管生成,从而导致肿瘤血管抑制剂血管抑素的释放。编码的蛋白质已被确定为月光蛋白质,基于其执行机械上不同功能的能力。它的缺乏与广泛的临床表型溶血性贫血和神经功能障碍有关。该基因的假基因已在19号,21号染色体和 X 染色体上被定义。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人磷酸甘油酸酯激酶1(PGK1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人磷酸甘油酸酯激酶1(PGK1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human PGK1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human PGK1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.