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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
FABP1是一个基因,编码蛋白质产品FABP1(脂肪酸结合蛋白1),也经常被称为肝脏型脂肪酸结合蛋白(LFABP)。FABP1主要在肝脏中表达,参与长链脂肪酸(LCFAs)、内源性大麻素、植物性大麻素(对合成的大麻素受体(CBR)激动剂和拮抗剂的作用较小)和其他疏水性分子的结合、运输和代谢。FABP1主要在肝脏中表达,参与长链脂肪酸(LCFAs)、内源性大麻素、植物性大麻素(对合成的大麻素受体(CBR)激动剂和拮抗剂的作用较小)和其他疏水性分子的结合、运输和代谢。每个FABP都对应于身体周围的特定器官/组织,它们在脂肪酸的吸收、运输和代谢中发挥作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人肝型脂肪酸结合蛋白(FABP1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人肝型脂肪酸结合蛋白(FABP1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human FABP1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human FABP1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.