BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
蛋白C又称自凝血酶IIA和血凝因子XIX，是一种酶原，其活化形式在调节抗凝血、炎症和细胞死亡以及维持人类和其他动物血管壁的通透性方面起着重要作用。活化蛋白C（APC）主要通过蛋白溶解蛋白质因子Va和因子VIIIa来完成这些操作。APC被归类为丝氨酸蛋白酶，因为它的活性部位含有一个丝氨酸残基。蛋白C由PROC基因编码，该基因位于2号染色体上。蛋白C的酶原形式是一种依赖维生素K的糖蛋白，在血浆中循环。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人蛋白C(PC)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人蛋白C(PC)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human PC. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human PC present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
危险品化学品经营许可证（带存储）