BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
补体受体2型（CR2），又称补体C3d受体、Epstein-Barr病毒受体和CD21（分化簇21），是一种由CR2基因编码的蛋白质。CR2参与补体系统。它与iC3b（C3b的非活性衍生物）、C3dg或C3d结合。B细胞在其表面表达CR2受体，使补体系统在B细胞的激活和成熟中发挥作用。补体受体2与CD19相互作用，在成熟的B细胞上，与CD81（TAPA-1）形成复合物。CR2-CD19-CD81复合物通常被称为B细胞共受体复合物，因为当B细胞受体结合抗原时，CR2通过附着的C3d（或iC3b或C3dg）结合到被吸收的抗原。这导致B细胞对抗原的反应大大增强。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人补体受体2(CD21)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人补体受体2(CD21)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human CD21. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human CD21 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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