计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
谷氧还蛋白3(GLRX3),也被称为硫氧还蛋白样蛋白2(TXNL2),是由GLRX3基因编码的蛋白质。该基因编码谷氧还蛋白家族的成员。编码的蛋白质与蛋白激酶C theta结合并调节其功能。它还可能抑制细胞凋亡并在细胞生长中发挥作用,它的表达可能是癌症的标志物。它的假基因位于染色体6和9的短臂上。已经观察到该基因的剪接转录变体。它与BOLA2一起,作为细胞溶质铁硫 (Fe-S) 簇组装因子,促进[2Fe-2S]簇插入细胞溶质蛋白的子集。它作为心脏肥大的关键负调节剂和正性肌力调节剂。它是血红蛋白成熟所需。它不具有任何甲状腺氧还蛋白活性,因为它缺乏催化活性所必需的保守基序。它已被证明可以与PRKCQ相互作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人谷氧还蛋白3(GLRX3)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人谷氧还蛋白3(GLRX3)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human GLRX3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human GLRX3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.