BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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赖氨酸特异性组蛋白去甲基化酶1A（LSD1）也被称为赖氨酸（K）特异性去甲基化酶1A（KDM1A），是一种蛋白质，由KDM1A基因编码。LSD1是一种黄素依赖性单胺氧化酶，可以使单甲基化和双甲基化的赖氨酸，特别是组蛋白3、赖氨酸4和9（H3K4和H3K9）脱甲基。这种酶在胚胎发育和组织特异性分化以及卵母细胞生长中具有关键作用。KDM1A是第一个被发现的组蛋白去甲基化酶，尽管已经描述了30多个。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人赖氨酸特异性组蛋白去甲基化酶1(LSD1)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人赖氨酸特异性组蛋白去甲基化酶1(LSD1)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human LSD1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human LSD1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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