计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
载脂蛋白A1是一种蛋白质,由APOA1基因编码。作为高密度脂蛋白颗粒的主要成分,它在脂质代谢中具有特殊作用。APOA1基因位于第11条染色体上,具体位置为11q23-q24,该基因包含4个外显子。APOA1编码一个28.1kDa的蛋白质,由243个氨基酸组成;通过质谱数据观察到21个肽。载脂蛋白A1是血浆中高密度脂蛋白颗粒的主要蛋白成分。从肠道细胞分泌的乳糜微粒也含有载脂蛋白A1,但它在血液中迅速转移到高密度脂蛋白。该蛋白作为高密度脂蛋白颗粒的一个组成部分,通过接受细胞内的脂肪,使脂肪分子外流到其他地方,包括回到低密度脂蛋白颗粒或到肝脏排泄出来。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人载脂蛋白A1(Apo-A1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人载脂蛋白A1(Apo-A1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human Apo-A1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human Apo-A1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.