BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
载脂蛋白A5(ApoA-5)是一种蛋白质，由APOA5基因编码。它在肝脏中显著表达。它是一种载脂蛋白，是血浆甘油三酯水平的重要决定因素，是冠状动脉疾病的主要危险因素。它是几个脂蛋白部分的组成部分，包括VLDL、HDL、乳糜微粒。它通过与LDL-R基因家族受体相互作用而影响脂蛋白代谢。考虑到其与脂蛋白水平的关系，它与代谢综合征有关。APOA5基因还包含与冠状动脉疾病风险增加有关的27个SNP之一。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人载脂蛋白A5(ApoA-5)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人载脂蛋白A5(ApoA-5)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human ApoA-5. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human ApoA-5 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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