计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
丙二醛(MDA)是标称式为 CH2(CHO)2 的有机化合物。它是一种无色液体,是一种以烯醇形式出现的高活性化合物。它天然存在,是氧化压力的标志。它是由多不饱和脂肪酸的脂质过氧化产生的。它是血栓素 A2 合成的主要产品,其中环氧化酶 1 或环氧化酶 2 将花生四烯酸代谢成血小板和其他多种细胞类型和组织的前列腺素 H2。脂质过氧化的程度可以通过组织中丙二醛的数量来估计。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有丙二醛(MDA)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的丙二醛(MDA)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with MDA antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of MDA in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.