BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
淀粉酶是一种催化淀粉（amylase）水解成糖的酶。淀粉酶存在于一些哺乳动物的唾液中,它一般作用于可溶性淀粉、直链淀粉、糖原等α-1,4-葡聚糖，水解α-1,4-糖苷键的酶。根据作用的方式可分为α-淀粉酶与β-淀粉酶。α-淀粉酶广泛分布于动物、植物及微生物。微生物的酶几乎都是分泌性的。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有大鼠淀粉酶(AMS)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠淀粉酶(AMS)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Rat AMS antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Rat AMS in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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