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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
α淀粉酶(AMS)是一种的酶,可以水解大型的、α-连接的多糖(如淀粉和糖 原)的α键,产生其较短的链、糊精和麦芽糖。它是糖苷水解酶家族 13 的成 员。虽然在许多组织中发现,但淀粉酶在胰液和唾液中最为突出,每一种都 有自己的α-淀粉酶的同工酶。另外,所有的淀粉酶异构体都与染色体 1p21 相连。淀粉酶存在于唾液中,将淀粉分解成麦芽糖和糊精。这种形式的淀粉 酶也被称为"ptyalin"。它将把大的、不溶性的淀粉分子分解成可溶性的淀粉 (直链淀粉、赤链淀粉和链烷醇),产生连续的小淀粉,最终产生麦芽糖。 Ptyalin 作用于线性α(1,4)糖苷连接,但复合水解需要一种作用于支链产物的 酶。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有小鼠α淀粉酶(α-AMS)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠α淀粉酶(α-AMS)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Mouse α-AMS antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Mouse α-AMS in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.