BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
脂多糖，现在更多地被称为内毒素，是革兰氏阴性细菌（如大肠杆菌和沙门氏菌）细胞膜最外层具有共同结构构造的成分的总称。 脂多糖（LPS）是一种大分子，由三部分组成：被称为 O 抗原的外核多糖、内核寡糖和脂质 A（毒性主要来自脂质 A），三者共价连接。 LPS 是免疫系统的强效激活剂，也是一种热原（引起发烧的物质）。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有小鼠脂多糖(LPS)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠脂多糖(LPS)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Mouse LPS antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Mouse LPS in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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