荧光抗体稀释液用于荧光染色时一抗 (primary antibody)、二抗 (secondary antibody)的稀释和配制,荧光抗体的染色背景如果较高,可以用荧光抗体稀释液 (Evans Blue法)稀释至临界浓度,使特异性染色呈阳性而非特异性染色呈阴性。
荧光抗体稀释液 (Evans Blue法)由伊文思蓝、盐等组成,为蓝色液体,减少非特异性荧光,宜做常规应用。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
自备材料:
载玻片、盖玻片、荧光显微镜
操作步骤(仅供参考):
按实验具体要求操作,稀释抗体后应4℃避光保存。
注意事项:
1、荧光物质均易发生淬灭,染色后的样品宜避光保存。
2、在使用荧光抗体稀释液的情况下,虽然可减缓淬灭,仍需尽量避光。
3、试剂开封后请尽快使用,以防影响后续实验效果。
Fluorescent Antibody Diluent is used for the dilution and preparation of primary antibodies and secondary antibodies in fluorescent staining. If the staining background of fluorescent antibodies is relatively high, Fluorescent Antibody Diluent (Evans Blue Method) can be used to dilute the antibodies to the critical concentration, which ensures positive results for specific staining and negative results for non-specific staining.
Fluorescent Antibody Diluent (Evans Blue Method) is composed of Evans Blue, salts, and other components. It is a blue liquid that reduces non-specific fluorescence and is suitable for routine applications. This reagent is for research use only and not intended for clinical diagnosis or other purposes.
Materials to Be Prepared by the User
Glass slides, coverslips, fluorescence microscope
Operating Procedures (for reference only)
Operate according to the specific requirements of the experiment. After antibody dilution, store the diluted solution at 4°C in the dark.
Precautions
1. All fluorescent substances are prone to quenching; stained samples should be stored in the dark.
2. Although the use of Fluorescent Antibody Diluent can mitigate quenching, it is still necessary to avoid light exposure as much as possible.
3. Please use the reagent as soon as possible after opening to avoid affecting the results of subsequent experiments.