BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
胆固醇(Cholesterol)又称胆甾醇，是一种环戊烷多氢菲的衍生物，广泛存在于动物体内，其中脑、神经组织最丰富，在肾、脾、皮肤、肝和胆汁中含量也较高，用酶学方法测定游离胆固醇(FC)是生化检测中的常用方法。 血液中的胆固醇约1/3为游离胆固醇，2/3为与脂肪酸结合的胆固醇酯，后者被胆固醇酯酶(CEH)水解为游离胆固醇，游离胆固醇被胆固醇氧化酶(COD)氧化成胆甾烯酮，并产生过氧化氢，再经过氧化物酶(POD)催化，使4-氨基安替比林与酚(三者合称PAP)反应，生成红色醌亚胺色素(Trinder反应)，当CEH不存在时胆固醇酯不能被水解，因而只能检测到游离胆固醇的含量，分光光度计在500~520nm处进行比色测定。 该试剂盒主要用于人或动物的血清、血浆、脑脊液、细胞、组织等样本中的游离胆固醇含量定量测定。仅用于科研领域，不适用于临床诊断或其他用途。
Cholesterol, also known as cholesterin, is a derivative of cyclopentanoperhydrophenanthrene. It is widely distributed in animals, with the highest concentrations found in the brain and nerve tissues. It is also relatively abundant in the kidneys, spleen, skin, liver, and bile. The enzymatic method for determining Free Cholesterol (FC) is a common technique in biochemical assays. In blood, approximately one-third of cholesterol exists as free cholesterol, while the remaining two-thirds is in the form of cholesterol esters bound to fatty acids. These cholesterol esters are hydrolyzed into free cholesterol by cholesterol esterase (CEH). The free cholesterol is then oxidized by cholesterol oxidase (COD) to produce cholesterone and hydrogen peroxide. Subsequently, in the presence of peroxidase (POD), the hydrogen peroxide catalyzes the reaction between 4-aminoantipyrine and phenol (collectively referred to as PAP), generating a red quinoneimine dye (Trinder reaction). When CEH is absent, cholesterol esters cannot be hydrolyzed, allowing for the measurement of free cholesterol content only. Absorbance is measured using a spectrophotometer at wavelengths between 500 nm and 520 nm. This assay kit is primarily designed for the quantitative determination of free cholesterol in samples such as human or animal serum, plasma, cerebrospinal fluid, cells, and tissues. It is intended for research use only and is not suitable for clinical diagnosis or other purposes.
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