果糖-6-磷酸(F6P )是糖代谢和糖异生途径的中间产物 ,广泛地存在于动植物和微生物体中。
本试剂盒提供一种简单,灵敏,快速的测定方法:果糖-6-磷酸(F6P)在磷酸葡萄糖异构酶和磷酸葡萄糖脱氢酶的相继作用下使NADP⁺还原成NADPH,通过检测NADPH在340nm处的增加量即可计算得出样品中的F6P含量。
Fructose-6-phosphate (F6P) is an intermediate product in the glycolysis and gluconeogenesis pathways and is widely present in animals, plants, and microorganisms.
This kit provides a simple, sensitive, and rapid assay method: Fructose-6-phosphate (F6P) is converted under the sequential action of phosphoglucose isomerase and glucose-6-phosphate dehydrogenase, reducing NADP⁺ to NADPH. By measuring the increase in NADPH at 340 nm, the F6P content in the sample can be calculated.